Structure of the carbohydrate recognition domain of Dectin-2 complexed with a mammalian-type high mannose Man9GlcNAc2 oligosaccharide

Mechanism of pathogen recognition by human dectin-2.

Liquid nitrogen-cooled double crystal Si(111)

the N-terminal ALA is a result of cloning

C-type lectin superfamily member 10,Dendritic cell-associated C-type lectin 2,Dectin-2

ALTCFSEGTKVPAWGCCPASWKSFGSSCYFISSEEKVWSKSEQNCVEMGAHLVVFNTEAEQNFIVQQLNESFSYFLGLSD
PQGNNNWQWIDKTPYEKNVRFWHLGEPNHSAEQCASIVFWKPTGWGWNDVICETRRNSICEMNKIYL

Drop size:
0.9:0.9 micro-liter of protein:reservoir
protein solution:
2 mg/ml CRD, 5 mM CaCl2, 10 mM Tris-Cl, pH 8.0, 25 mM NaCl, and 2 mM Man9GlcNAc2.
The reservoir solution:
1.0 M NaCl, 6% polyethylene glycol 400 and 0.1 M Tris-Cl, pH 8.0.
freezing solution:
30% polyethylene glycol 400, 1.0 M NaCl, 0.1 M Tris-Cl, pH 8.0, and 2 mM Man9GlcNAc2.

Biotechnology and Biological Sciences Research Council (BBSRC)

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

custom non-bonded symmetry exclusions added for the mannose residues

carbohydrate-binding protein; lectin; glycoprotein, SUGAR BINDING PROTEIN

LTCFSEGTKVPAWGCCPASWKSFGSSCYFISSEEKVWSKSEQNCVEMGAHLVVFNTEAEQNFIVQQLNESFSYFLGLSDP
QGNNNWQWIDKTPYEKNVRFWHLGEPNHSAEQCASIVFWKPTGWGWNDVICETRRNSICEMNKIYL