Y316F mutant of corn root ferredoxin:NADP+ reductase

High-resolution studies of hydride transfer in the ferredoxin:NADP(+) reductase superfamily.

Atom 'A SER 129 CB' has occupancy 2.00. Atom 'A SER 129 OG' has occupancy 2.00. Atom 'A SER 129 HB2' has occupancy 2.00. Atom 'A SER 129 HB3' has occupancy 2.00. Atom 'A SER 129 HG' has occupancy 2.00.

SVQQASRSKVSVAPLHLESAKEPPLNTYKPKEPFTATIVSVESLVGPKAPGETCHIVIDHGGNVPYWEGQSYGVIPPGEN
PKKPGAPQNVRLYSIASTRYGDNFDGRTGSLCVRRAVYYDPETGKEDPSKNGVCSNFLCNSKPGDKIQLTGPSGKIMLLP
EEDPNATHIMIATGTGVAPFRGYLRRMFMEDVPNYRFGGLAWLFLGVANSDSLLYDEEFTSYLKQYPDNFRYDKALSREQ
KNRSGGKMYVQDKIEEYSDEIFKLLDGGAHIYFCGLKGMMPGIQDTLKKVAERRGESWDQKLAQLKKNKQWHVEVF

22-24% PEG 8000, 0.1 M sodium cacodylate (pH 6-7), 0.18-0.22 M magnesium acetate, 100 mM nicotinamide

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Science Foundation (NSF, United States)

flavoenzyme, hydride transfer, photosynthesis, active site compression, TRANSFERASE, OXIDOREDUCTASE

SVQQASRSKVSVAPLHLESAKEPPLNTYKPKEPFTATIVSVESLVGPKAPGETCHIVIDHGGNVPYWEGQSYGVIPPGEN
PKKPGAPQNVRLYSIASTRYGDNFDGRTGSLCVRRAVYYDPETGKEDPSKNGVCSNFLCNSKPGDKIQLTGPSGKIMLLP
EEDPNATHIMIATGTGVAPFRGYLRRMFMEDVPNYRFGGLAWLFLGVANSDSLLYDEEFTSYLKQYPDNFRYDKALSREQ
KNRSGGKMYVQDKIEEYSDEIFKLLDGGAHIYFCGLKGMMPGIQDTLKKVAERRGESWDQKLAQLKKNKQWHVEVY