Structure of wild type pre-miR21 apical loop
A Macrocyclic Peptide Ligand Binds the Oncogenic MicroRNA-21 Precursor and Suppresses Dicer Processing.
Sample conditions to identify exchangeable protons and 15N chemical shifts. Low temperatures were used to reduce base-pair breathing and exchange with solvent. The RNA sample was snapped cooled prior to NMR data collection (heated 95C for 4min then at -20C until frozen, sample was thawed on ice prior to data collection)
Sample conditions to identify non-exchangeable protons and 13C chemical shifts. The RNA sample was snapped cooled prior to NMR data collection (heated 95C for 4min then at -20C until frozen, sample was thawed on ice prior to data collection)
1.1 mM pre-miR-21, 10 mM potassium phosphate, 10 mM sodium chloride, 0.01 mM EDTA, 95% H2O/5% D2O
1.1 mM pre-miR-21, 10 mM potassium phosphate, 10 mM sodium chloride, 0.01 mM EDTA, 100% D2O
0.5 mM [U-99% 13C; U-99% 15N] pre-miR-21, 10 mM potassium phosphate, 10 mM sodium chloride, 0.01 mM EDTA, 100% D2O
0.5 mM [U-99% 13C; U-99% 15N] pre-miR-21, 10 mM potassium phosphate, 10 mM sodium chloride, 0.01 g/L EDTA, 95% H2O/5% D2O
Schwieters, Kuszewski, Tjandra and Clore
Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax