Crystal structure of SARO_2595 from Novosphingobium aromaticivorans

Novosphingobium aromaticivoransuses a Nu-class glutathioneS-transferase as a glutathione lyase in breaking the beta-aryl ether bond of lignin.

SAIAGMSSEYVPPKVWKWDKANGGAFASVNRPVAGPTSERELPVGKHPFQVYSLGTPNGQKATIMLEELLQLGFSEAEYD
AWLIKIFEGDQFTSGFVDINPNSKIPAMVDRSGPEPFRVFESGAILMHLAEKFGVFLPTSGPARAECLSWLFWQVGSAPF
IGGGFGHFYNYAPIKIEYAIDRYAMETKRLFDVANRRLAESRYLAGDEYTIADLATYTWFGNIYRGEAYGEAATFLSMHE
YEHVGRWVGEIDARPGVLRGRLVNSSKGLAERHDASDFDALPPESLQAIVKGF

Novosphingobium aromaticivorans (strain ATCC 700278 / DSM 12444 / CIP 105152 / NBRC 16084 / F199)

Saro protein at 277 mM was incubated with 10 mM reduced glutathione for 50 minutes prior to setting up crystallization experiments. The droplet yielding the crystal with the highest observed diffracting power was composed of 130 nL protein and 85 nL reservoir solution.
The reservoir solution was
1.35 M ammonium sulfate,
0.1 M lithium sulfate
0.1 M bis-trispropane, pH 7.5

Department of Energy (DOE, United States)

Bioenergy, GLBRC, lignin valorization, TRANSFERASE

MSSEYVPPKVWKWDKANGGAFASVNRPVAGPTSERELPVGKHPFQVYSLGTPNGQKATIMLEELLQLGFSEAEYDAWLIK
IFEGDQFTSGFVDINPNSKIPAMVDRSGPEPFRVFESGAILMHLAEKFGVFLPTSGPARAECLSWLFWQVGSAPFIGGGF
GHFYNYAPIKIEYAIDRYAMETKRLFDVANRRLAESRYLAGDEYTIADLATYTWFGNIYRGEAYGEAATFLSMHEYEHVG
RWVGEIDARPGVLRGRLVNSSKGLAERHDASDFDALPPESLQAIVKGF