Structure of RNF165 in complex with a UbcH5b~Ub conjugate

Discovery of new non-covalent ubiquitin binding sites on UbcH5

(E3-independent) E2 ubiquitin-conjugating enzyme D2,E2 ubiquitin-conjugating enzyme D2,Ubiquitin carrier protein D2,Ubiquitin-conjugating enzyme E2(17)KB 2,Ubiquitin-conjugating enzyme E2-17 kDa 2,Ubiquitin-protein ligase D2,p53-regulated ubiquitin-conjugating enzyme 1

GPLGSMALKRIHKELNDLARDPPAQSSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYH
PNINSNGSIKLDILRSQWSPALTISKVLLSISSLLSDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM

GPLGSGAVQNTIERFTFPHKYKKGKGKKDEGEESDTDEKCTICLSMLEDGEDVRRLPCAHLFHQLCVDQWLAMSKKCPIC
RVDIETQLGADS

UBE2D2, PUBC1, UBC4, UBC5B, UBCH4, UBCH5B

200 mM potassium thiocyanate, 20% PEG 3350

U VALUES : WITH TLS ADDED HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Open conformation, Backside, Isopeptide-linked, TRANSFERASE

MALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINS
NGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAM

GAVQNTIERFTFPHKYKKRRPQDGKGKKDEGEESDTDEKCTICLSMLEDGEDVRRLPCMHLFHQLCVDQWLAMSKKCPIC
RVDIETQLGADS