Zinc-Binding Structure of a Catalytic Amyloid from Solid-State NMR Spectroscopy
Zinc-binding structure of a catalytic amyloid from solid-state NMR.
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)
National Institutes of Health/National Institute on Aging (NIH/NIA)
0.4 g/L [U-13C; U-15N] IVL, pH 8 Tris buffer
13C, 15N-labeled IVL fibril sample with Zn2+
0.4 g/L [U-13C] mixed sample, pH 8 Tris buffer
Mixed labeled fibrils (samples 3, 4, 9 and 10) for determining intermolecular packing were prepared using a urea-based solubilization protocol to ensure complete mixing.
Schwieters, Kuszewski, Tjandra and Clore