Characterization of Two Late-Stage Enzymes Involved in Fosfomycin Biosynthesis in Pseudomonads.

(S)-2-hydroxypropylphosphonic acid epoxidase

Hydroxypropylphosphonate epoxidase,Ps-hppE

MDVRTLAVGKAHLEALLATRKMTLEHLQDVRHDATQVYFDGLEHLQNVAQYLAIPLSEFFVGQTQSDLDDGVKIARRNGG
FKREEIRGGVHYYTYEHLVTTNQDPGLMALRLDLHSDDEQPLRLNGGHGSREIVYVTRGAVRVRWVGDNDELKEDVLNEG
DSIFILPNVPHSFTNHVGGAKSEIIAINYG

22% PEG 3,350
0.1 M Ammonium Citrate
10 mg/mL Psf4
Crystallized Anaerobically

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Fosfomycin, Epoxidase, Oxidase, OXIDOREDUCTASE