Crystal structure of Staphylococcus epidermidis Aap G58-spacer-G513 (variant G5-spacer-consensus G5)

Functional consequences of B-repeat sequence variation in the staphylococcal biofilm protein Aap: deciphering the assembly code.

Accumulation associated protein Aap G58-spacer-G513 (variant G5-spacer-consensus G5)

GVDGDPITSTEEIPFDKKREFNPDLKPGEERVKQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGE
EIKPGHKDEFDPNAPKGSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGPVDGDSITSTEEIPFDKKREFDPNLAPGTEKV
VQKGEPGTKTITTPTTKNPLTGEKVGEGKSTEKVTKQPVDEIVEYGPT

Staphylococcus epidermidis (strain ATCC 35984 / RP62A)

0.1M sodium acetate pH 5.0
0.2 M calcium chloride
25% PEG 6000

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

the structure was refined in both BUSTER and PHENIX

biofilm intercellular adhesion Staphylococcus Aap, PROTEIN BINDING

VDGDPITSTEEIPFDKKREFNPDLKPGEERVKQKGEPGTKTITTPTTKNPLTGEKVGEGEPTEKITKQPVDEITEYGGEE
IKPGHKDEFDPNAPKGSQEDVPGKPGVKNPDTGEVVTPPVDDVTKYGP