LIGAND BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA PAO1 AMINO ACID CHEMORECEPTOR PCTA IN COMPLEX WITH L-TRP

How Bacterial Chemoreceptors Evolve Novel Ligand Specificities

Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids.

Methyl-accepting chemotaxis protein PctA,Uncharacterized protein

MGSSHHHHHHSSGLVPRGSHMNDYLQRNAIREDLESYLREMGDVTSSNIQNWLGGRLLLVEQTAQTLARDHSPETVSALL
EQPALTSTFSFTYLGQQDGVFTMRPDSPMPAGYDPRSRPWYKDAVAAGGLTLTEPYVDAATQELIITAATPVKAAGNTLG
VVGGDLSLKTLVQIINSLDFSGMGYAFLVSGDGKILVHPDKEQVMKTLSEVYPQNTPKIATGFSEAELHGHTRILAFTPI
KGLPSVTWYLALSIDKDKAYAMLSKFRVSA

COUNTERDIFFUSION METHOD:
2.0 M SODIUM FORMATE, 0.1 M NA ACT pH 4.6

Same protein complexed with tryptophane.

SIGNALING PROTEIN, CHEMOTACTIC TRANSDUCER

NDYLQRNAIREDLESYLREMGDVTSSNIQNWLGGRLLLVEQTAQTLARDHSPETVSALLEQPALTSTFSFTYLGQQDGVF
TMRPDSPMPAGYDPRSRPWYKDAVAAGGLTLTEPYVDAATQELIITAATPVKAAGNTLGVVGGDLSLKTLVQIINSLDFS
GMGYAFLVSGDGKILVHPDKEQVMKTLSEVYPQNTPKIATGFSEAELHGHTRILAFTPIKGLPSVTWYLALSIDKDKAYA
MLSKFRVSA