Crystal Structure of Kemp Eliminase HG3.17 in unbound state, 277K

Ensemble-based enzyme design can recapitulate the effects of laboratory directed evolution in silico.

MAEAAQSIDQLIKARGKVYFGVATDQNRLTTGKNAAIIKADFGMVWPEESMQWDATEPSQGNFNFAGADYLVNWAQQNGK
LIGAGCLVWHNFLPSWVSSITDKNTLINVMKNHITTLMTRYKGKIRTWDVVGEAFNEDGSLRQNVFLNVIGEDYIPIAFQ
TARAADPNAKLYIMDYNLDSASYPKTQAIVNRVKQWRAAGVPIDGIGSQMHLSAGQGAGVLQALPLLASAGTPEVSILML
DVAGASPTDYVNVVNACLNVQSCVGITVMGVADPDSAFASSTPLLFDGNFNPKPAYNAIVQNLQQGSIEGRGHHHHHH

1.8-2.4 M Ammonium Sulfate, 4mg/mL Protein Concentration, 100mM Sodium Acetate

Natural Sciences and Engineering Research Council of Canada

Crystal structure of Kemp eliminase HG3.17 unbound state, 277K
Crystal structures of HG-series of Kemp Eliminases at Room-temperature

QAAQSVDQLIKARGKVYFGVATDQNRLTTGKNAAIIQADFGQVTPENSMKWDATEPSQGNFNFAGADYLVNWAQQNGKLI
RGHTLVWHSQLPSWVSSITDKNTLTNVMKNHITTLMTRYKGKIRAWDVVNEAFNEDGSLRQTVFLNVIGEDYIPIAFQTA
RAADPNAKLYINDYNLDSASYPKTQAIVNRVKQWRAAGVPIDGIGSQTHLSAGQGAGVLQALPLLASAGTPEVAITELDV
AGASPTDYVNVVNACLNVQSCVGITVWGVADPDSWRASTTPLLFDGNFNPKPAYNAIVQDLQQ