The cryofrozen atomic resolution X-ray crystal structure of the reduced form (Fe2+) perdeuterated Pyrococcus furiosus Rubredoxin in D2O (100K, 0.75 Angstrom resolution)
Cryostream frozen (never flash frozen in liquid N2)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
bipyramid bright red (Fe3+), Subsequently reduced with Na2S2O3 to get transparent (Fe2+) crystal. X-ray data collection performed with helicoidal strategy over 180 degrees rotation.
SEEDED 3.4M EQUIMOLAR NA/K PHOSPHATE BUFFER
Engineering and Physical Sciences Research Council
obsolete X-ray lower resolution reduced form (Fe2+) structure
Xray structure of the oxidised form (Fe3+)
Neutron structure of the oxidised form (Fe3+)
Deuterium atoms are isotropic, protein atoms, Fe atom and solvent O atoms are anisotropic
Perdeuterated rubredoxin, pyrococcus furiosus, atomic resolution, cryofrozen, reduced, iron, ELECTRON TRANSPORT