Apo HcgC from Methanococcus maripaludis soaked with SAH and pyridinol

A Water-Bridged H-Bonding Network Contributes to the Catalysis of the SAM-Dependent C-Methyltransferase HcgC.

MNYGITESVKTTRSKIKIKDIVSDVVEKKANAIKYFLEGEEFKQAIVFGAYLSGSYIAYSLLKDCEEVIIVDIQPHLKDI
LFNDGIKFMDLNKLQLELRNGTSINPDLVIDLTGIGGVSPDLISKFNPKVLIVEDPKGNHDKGISKIDNTDKRLCVGAKK
GVLKTYRSSKFSKTSGTMTLVVDIIMDSCREINELDSVLYTIPNLKYFEGTVFHEKNVKKFLTELNMSAITVSSIDHVEY
ELEEILSKNISRVDSFVKEFDKLAAALEHHHHHH

The drop consists of 1 ul of enzyme solution containing 5 mg/ml HcgC mixed with 1 ul of the reservoir solution : 100 mM HEPES/NaOH pH 7.5, 0.1 M NaCl, and 30% MPD (2-Methyl-2,4-pentanediol). The soaking was performed overnight in the same crystallization buffer containing 2 mM SAH and 3 mM pyridinol (dissolved in 100% DMSO).

methyltransferases, biosynthesis, protein structures, enzyme catalysis, mutagenesis, [Fe]-hydrogenase, pyridinol, Hmd, Transferase

MNYGITESVKTTRSKIKIKDIVSDVVEKKANAIKYFLEGEEFKQAIVFGAYLSGSYIAYSLLKDCEEVIIVDIQPHLKDI
LFNDGIKFMDLNKLQLELRNGTSINPDLVIDLTGIGGVSPDLISKFNPKVLIVEDPKGNHDKGISKIDNTDKRLCVGAKK
GVLKTYRSSKFSKTSGTMTLVVDIIMDSCREINELDSVLYTIPNLKYFEGTVFHEKNVKKFLTELNMSAITVSSIDHVEY
ELEEILSKNISRVDSFVKEF