Crystal Structure of mutant M54L/M64L/M96L of Two-Domain Laccase from Streptomyces griseoflavus dialyzed against solution containing 0.25 mM copper sulfate

Incorporation of Copper Ions into T2/T3 Centers of Two-Domain Laccases.

MDRRGFNRRVLLGGVAATTSLSIAPEAVSAPESAGTAAAAGAAPAGGEVRRVTLYAERLAGGQLGYGLEKGKASIPGPLI
ELNEGDTLHVEFENTLDVPVSLHVHGLDYEISSDGTKQNKSHVEPGGTRTYTWRTHEPGRRADGTWRAGSAGYWHYHDHV
VGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFNDMTINNRPAHTGPNFEATVGDRVEIVMITHGEYYHTFHMHGHR
WADNRTGMLTGPDDPSQVIDNKICGPADSFGFQIIAGEGVGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYDPQEH
AH

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Two-Domain Laccase, laccase, Streptomyces griseoflavus, oxidoreductase

MDRRGFNRRVLLGGVAATTSLSIAPEAVSAPESAGTAAAAGAAPAGGEVRRVTMYAERLAGGQMGYGLEKGKASIPGPLI
ELNEGDTLHVEFENTMDVPVSLHVHGLDYEISSDGTKQNKSHVEPGGTRTYTWRTHEPGRRADGTWRAGSAGYWHYHDHV
VGTEHGTGGIRNGLYGPVIVRRKGDVLPDATHTIVFNDMTINNRPAHTGPNFEATVGDRVEIVMITHGEYYHTFHMHGHR
WADNRTGMLTGPDDPSQVIDNKICGPADSFGFQIIAGEGVGAGAWMYHCHVQSHSDMGMVGLFLVKKPDGTIPGYDPQEH
AH