Crystal structure of the complex between Nb474 and Trypanosoma congolense fructose-1,6-bisphosphate aldolase

Structural basis for the high specificity of a Trypanosoma congolense immunoassay targeting glycosomal aldolase.

MSRRVEVLLTQLPAYNRLKTPYEEELIETAKKMTAPGKGLLAADESTGSCSKRFAGIGLSNTAEHRRQYRALMLECAGFE
QYISGVILHDETVYQRASTGETFPQLLRRRGVVPGIKTDCGLEPLVEGADGEQMTAGLDGYVKRAKKYYAVGCRFCKWRN
VYKIQNGTVSEAVVRFNAETLARYAVLSQLCGLVPIVEPEVMIDGTHDIETCQRVSQHVWAEVVSALHRHGVVWEGCLLK
PNMVVPGAESGQTATAEQVAEYTVKTLARVLPPALPGVTFLSGGLSEVMASEYLNAMNNSPLPRPWKLTFSYARALQSSA
IKAWGGKSSGVAAGRRAFMHRAKMNSLAQLGRYNRGDDDKDSQSLYVAGNTYENLYFQSGGHHHHHH

QVQLQESGGGLVQPGGSLRLSCAASETALTYYAIGWFRQAPGKEREGVSCISRINSGSGARTDYADSVKGRFTISRDDAK
NTVTLQMNSLEPEDTARYYCALDTTDRYDSANGRYYCTISSDTYDYWGQGTQVTVSSHHHHHH

100 mM sodium cacodylate pH 6.5, 200 mM magnesium acetate, 10% PEG 8000

complex, Nanobody, aldolase, diagnosis, lyase

(chain A and ((resid 2 through 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 18 or (resid 19 and (name N or name CA or name C or name O or name CB )) or resid 20 through 23 or (resid 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 27 or (resid 28 and (name N or name CA or name C or name O or name CB )) or resid 29 through 31 or (resid 32 and (name N or name CA or name C or name O or name CB or name CG )) or resid 33 through 52 or (resid 53 and (name N or name CA or name C or name O or name CB or name CG )) or resid 54 through 107 or (resid 108 and (name N or name CA or name C or name O or name CB or name CG )) or resid 109 through 142 or (resid 143 and (name N or name CA or name C or name O or name CB or name CG )) or resid 144 through 146 or (resid 147 and (name N or name CA or name C or name O or name CB or name CG )) or resid 148 through 321 or (resid 322 through 323 and (name N or name CA or name C or name O or name CB )) or resid 324 through 326 or (resid 327 and (name N or name CA or name C or name O or name CB )) or resid 328 through 359))

(chain B and ((resid 2 through 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 23 or (resid 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 27 or (resid 28 and (name N or name CA or name C or name O or name CB )) or resid 29 through 30 or (resid 31 and (name N or name CA or name C or name O or name CB )) or (resid 32 and (name N or name CA or name C or name O or name CB or name CG )) or resid 33 through 52 or (resid 53 and (name N or name CA or name C or name O or name CB or name CG )) or resid 54 through 107 or (resid 108 and (name N or name CA or name C or name O or name CB or name CG )) or resid 109 through 142 or (resid 143 and (name N or name CA or name C or name O or name CB or name CG )) or resid 144 through 326 or (resid 327 and (name N or name CA or name C or name O or name CB )) or resid 328 through 351 or (resid 352 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 353 through 359))

(chain C and ((resid 2 through 3 and (name N or name CA or name C or name O or name CB )) or resid 4 through 23 or (resid 24 and (name N or name CA or name C or name O or name CB )) or resid 25 through 30 or (resid 31 and (name N or name CA or name C or name O or name CB )) or resid 32 through 52 or (resid 53 and (name N or name CA or name C or name O or name CB or name CG )) or resid 54 through 146 or (resid 147 and (name N or name CA or name C or name O or name CB or name CG )) or resid 148 through 321 or (resid 322 through 323 and (name N or name CA or name C or name O or name CB )) or resid 324 through 326 or (resid 327 and (name N or name CA or name C or name O or name CB )) or resid 328 through 351 or (resid 352 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 353 through 359))

(chain D and (resid 2 through 18 or (resid 19 and (name N or name CA or name C or name O or name CB )) or resid 20 through 30 or (resid 31 and (name N or name CA or name C or name O or name CB )) or resid 32 through 107 or (resid 108 and (name N or name CA or name C or name O or name CB or name CG )) or resid 109 through 142 or (resid 143 and (name N or name CA or name C or name O or name CB or name CG )) or resid 144 through 146 or (resid 147 and (name N or name CA or name C or name O or name CB or name CG )) or resid 148 through 321 or (resid 322 through 323 and (name N or name CA or name C or name O or name CB )) or resid 324 through 359))

(chain E and (resid 2 through 4 or (resid 5 and (name N or name CA or name C or name O or name CB )) or resid 6 through 10 or (resid 11 and (name N or name CA or name C or name O or name CB )) or resid 12 through 18 or (resid 19 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 20 through 42 or (resid 43 through 44 and (name N or name CA or name C or name O or name CB )) or resid 45 through 68 or (resid 69 and (name N or name CA or name C or name O or name CB )) or resid 70 through 90 or (resid 91 and (name N or name CA or name C or name O or name CB )) or resid 92 through 128 or (resid 129 and (name N or name CA or name C or name O or name CB )) or resid 130 through 137))

(chain F and (resid 2 through 18 or (resid 19 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 20 through 25 or (resid 26 and (name N or name CA or name C or name O or name CB )) or resid 27 through 43 or (resid 44 and (name N or name CA or name C or name O or name CB )) or resid 45 or (resid 46 and (name N or name CA or name C or name O or name CB )) or resid 47 through 68 or (resid 69 and (name N or name CA or name C or name O or name CB )) or resid 70 through 79 or (resid 80 and (name N or name CA or name C or name O or name CB )) or resid 81 through 128 or (resid 129 and (name N or name CA or name C or name O or name CB )) or resid 130 through 137))

(chain G and (resid 2 through 10 or (resid 11 and (name N or name CA or name C or name O or name CB )) or resid 12 through 18 or (resid 19 and (name N or name CA or name C or name O or name CB or name CG or name CD )) or resid 20 through 25 or (resid 26 and (name N or name CA or name C or name O or name CB )) or resid 27 through 42 or (resid 43 through 44 and (name N or name CA or name C or name O or name CB )) or resid 45 through 68 or (resid 69 and (name N or name CA or name C or name O or name CB )) or resid 70 through 79 or (resid 80 and (name N or name CA or name C or name O or name CB )) or resid 81 through 137))

(chain H and (resid 2 through 10 or (resid 11 and (name N or name CA or name C or name O or name CB )) or resid 12 through 42 or (resid 43 through 44 and (name N or name CA or name C or name O or name CB )) or resid 45 through 79 or (resid 80 and (name N or name CA or name C or name O or name CB )) or resid 81 through 128 or (resid 129 and (name N or name CA or name C or name O or name CB )) or resid 130 through 137))

MSRRVEVLLTQLPAYNRLKTPYEEELIETAKKMTAPGKGLLAADESTGSCSKRFAGIGLSNTAEHRRQYRALMLECAGFE
QYISGVILHDETVYQRASTGETFPQLLRRRGVVPGIKTDCGLEPLVEGADGEQMTAGLDGYVKRAKKYYAVGCRFCKWRN
VYKIQNGTVSEAVVRFNAETLARYAVLSQLCGLVPIVEPEVMIDGTHDIETCQRVSQHVWAEVVSALHRHGVVWEGCLLK
PNMVVPGAESGQTATAEQVAEYTVKTLARVLPPALPGVTFLSGGLSEVMASEYLNAMNNSPLPRPWKLTFSYARALQSSA
IKAWGGKSSGVAAGRRAFMHRAKMNSLAQLGRYNRGDDDKDSQSLYVAGNTY