Crystal structure of Burkholderia pseudomallei D-alanine-D-alanine ligase in complex with AMP

Burkholderia pseudomallei d-alanine-d-alanine ligase; detailed characterisation and assessment of a potential antibiotic drug target.

D-Ala-D-Ala ligase,D-alanylalanine synthetase

MSGIDPKRFGKVAVLLGGDSAEREVSLNSGRLVLQGLRDAGIDAHPFDPAQRPLAALKDEGFVRAFNALHGGYGENGQIQ
GALDFYGIRYTGSGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDYAARAQDIVAKLGVPLFVKPASEGSSVAVEK
VKSADALPAALEEAAKHDKIVIVEKSIEGGGEYTACIAADLDLPLIRIVPAGEFYDYHAKYIANDTQYLIPCGLDAAKEA
EFKRIARRAFDVLGCTDWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGIGYSELVVKVLSLTLD

0.05 to 0.3 M Li2SO4, 0.1 M Bis-Tris pH 5.5 and 15-30% (w/v) PEG 3350, ratio protein:reservoir 1:1

Defence Sciecne and Technology Laboratory