Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures.
MQVQLVESGGGLVQAGGSLRLSCVVSGSAVSDYAMGWYRQAPGKQRELVAAIYNSGRTNYVDSVKGRFTISKDNAKKTVY LQMNSLKPEDTADYFCNLLGATTMSNAVWGQGTQVTVSSHHHHHH
0.2 M Ammonium Sulfate, 0.1 M Hepes pH 7.5, 25% w/v PEG 3350
The Danish Research Council for Independent Research
Ig domain llama single domain antibody nanobody, immune system