Structure of a novel, fully Human Fab binding to Interleukin 1-beta.

MGSTAILALLLAVLQGVCAEVQLVESGAEVKKPGESLKISCKGSGYTFTSYWIGWVRQMPGKGLEWMGIIYPGDSDTRYS
PSFQGQVTISADKSISTAYLQWSSLKASDTAMYYCARFVSLDAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTA
ALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKRVEPKSC

MEAPAQLLFLLLLWLPDTTGEIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA
RFSGSGSGTDFTLTISSLEPEDFAVYYCQQRSNWLWTFGQGTKVEIKRSVAAPSVFIFPPSDEQLKSGTASVVCLLNNFY
PREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECENLYFQ
GHHHHHHHH

MAHHHHHHHHHHHHPAGENLYFQGAPVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKI
PVALGLKEKNLYLSCVLKDDKPTLQLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGG
TKGGQDITDFTMQFVSS

Protein mix:
10mg/ml Protein
10mM Imidazole buffer, pH=8.0
50mM NaCl

Reservoir:
18% PEG4000 (w/v),
0.1M Hepes pH=7.0,
10mM ATP,

Drop Ratio 1:1

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Fab 4AB007 H-chain, Fab 4AB007 L-chain, Interleukin-1 beta

Interleukin-1-beta, Fab 4AB007, Signaling protein

APVRSLNCTLRDSQQKSLVMSGPYELKALHLQGQDMEQQVVFSMSFVQGEESNDKIPVALGLKEKNLYLSCVLKDDKPTL
QLESVDPKNYPKKKMEKRFVFNKIEINNKLEFESAQFPNWYISTSQAENMPVFLGGTKGGQDITDFTMQFVSS

binding site for Di-peptide LYS V 55 and SER A 133