Extracellular domain of human CD83 - rhombohedral crystal form after UV-RIP (S-SAD data)
Crystal Structure of the Extracellular Domain of the Human Dendritic Cell Surface Marker CD83.
The first four residues (GSPG) are non-native residues of the linker which remains after the GST-tag was cleaved off. The third residue (P) was modeled as alanine due to missing electron density. The first and last two residues as well as the central region were not visible in the electron density maps.
hCD83,B-cell activation protein,Cell surface protein HB15
GSPGTPEVKVASSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIR NTTSSNSGTYRCTLQDPDGQRNLSGKVILRVTGSPA
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
0.2 microliter protein (39 mg/ml in ultrapure water) and 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) were mixed and equilibrated against 70 microliter of reservoir solution Crystals appeared after ~13 months
5MIX is the same protein, but a different crystal form (space group P 3 2 1)
5MJ0 is the same protein, but a different crystal form (space group I 4_1 3 2)
5MJ1 is the same protein in the same space group, but before UV-RIP experiment
Dendritic cell, receptor, immunoglobulin, immune system
APATPEVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRN TTSCNSGTYRCTLQDPDGQRNLSGKVILRVTGCPA