Extracellular domain of human CD83 - rhombohedral crystal form
Crystal Structure of the Extracellular Domain of the Human Dendritic Cell Surface Marker CD83.
The first four residues (GSPG) are non-native residues of the linker which remain after the GST-tag was cleaved off. The third residue (P) was modeled as alanine due to missing electron density. The first and last two residues as well as the central region were not visible in the electron density maps.
hCD83,B-cell activation protein,Cell surface protein HB15
GSPGTPEVKVASSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIR NTTSSNSGTYRCTLQDPDGQRNLSGKVILRVTGSPA
Escherichia coli 'BL21-Gold(DE3)pLysS AG'
0.2 microliter protein (39 mg/ml in ultrapure water) mixed with 0.4 microliter reservoir solution (0.2 M DL-malic acid (pH 7.0), 20% w/v PEG 3350) was equilibrated against 70 microliter of reservoir solution. Crystals appeared after ~ 13 months
5MIX is the same protein, but a different crystal form (P321)
5MJ0 is the same protein, but a different crystal form (I4_1 3 2)
Dendritic cell, receptor, immunoglobulin, immune system
APATPEVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRN TTSCNSGTYRCTLQDPDGQRNLSGKVILRVTGCPA