Crystal structure of carboxyl esterase 2 (TmelEST2) from mycorrhizal fungus Tuber melanosporum

A family of archaea-like carboxylesterases preferentially expressed in the symbiotic phase of the mycorrhizal fungus Tuber melanosporum.

MRFTILSVLAFSLPLVLAETPAPSCPVTNVTTPQLDPITQAYADAISSRPSLFAFPLPEIRDGYQSNVSDPSTEFTTKIL
SLPVGPTGNVTAYLYKPVSGEREKGKDLLPVIAYFHGGGWVFGGPKSYRGLITNLIRESGAAVFFVDYTLTPKVAYPVPN
EQCYAAVQWLLEHGEKLGVDPTNMGFGGDSAGGELSSSVSLLSIKRKTPLPKFQVLIYPATDLACESATFKEFPNGPGLT
TDEIRFAASLFTPDPKSRLEDVASPGRASDEDLAKFPETLIVVAEVDPIRQQGEDFGRRLQKLGVRAAIIRVLGTIHGFA
SIDVLSEAPGAKATIELIGYKFKKALH

18 mg/ml in 25 mM Tris-HCl pH 8.0, 0.1M NaCl, 2% v/v Triton X-100) and 20% polyethylene glycol 3350 in 0.2 M KNO3 (pH 6.0)

lipase alpha/beta hydrolase fold archaebacterial-like enzymes, Hydrolase