Structure of a bacterial light-regulated adenylyl cylcase

Photoactivation Mechanism of a Bacterial Light-Regulated Adenylyl Cyclase.

Beta subunit of photoactivated adenylyl cyclase
Beggiatoa photoactivatable adenylyl cyclase bPAC

GSHMMKRLVYISKISGHLSLEEIQRIGKVSIKNNQRDNITGVLLYLQGLFFQILEGENEKVDKLYKKILVDDRHTNILCL
KTEYDITDRMFPNWAMKTINLNENSELMIQPIKSLLQTITQSHRVLEKYMPARVIYLINQGINPLTVEPQLVEKIIFFSD
ILAFSTLTEKLPVNEVVILVNRYFSICTRIISAYGGEVTKFIGDCVMASFTKEQGDAAIRTSLDIISELKQLRHHVEATN
PLHLLYTGIGLSYGHVIEGNMGSSLKMDHTLLGDAVNVAARLEALTRQLPYALAFTAGVKKCCQAQWTFINLGAHQVKGK
QEAIEVYTVNEAQKYYDTLQITQLIRQTLENDK

0.1 M Na-MES pH 6.5, 0.6 M NaCl, 20 % w/v PEG 3350; 0.3 mM alpha,beta-methylene-ATP in drop

BLUF, adenylyl cyclase, photoreceptor, optogenetics, lyase

MMKRLVYISKISGHLSLEEIQRIGKVSIKNNQRDNITGVLLYLQGLFFQILEGENEKVDKLYKKILVDDRHTNILCLKTE
YDITDRMFPNWAMKTINLNENSELMIQPIKSLLQTITQSHRVLEKYMPARVIYLINQGINPLTVEPQLVEKIIFFSDILA
FSTLTEKLPVNEVVILVNRYFSICTRIISAYGGEVTKFIGDCVMASFTKEQGDAAIRTSLDIISELKQLRHHVEATNPLH
LLYTGIGLSYGHVIEGNMGSSLKMDHTLLGDAVNVAARLEALTRQLPYALAFTAGVKKCCQAQWTFINLGAHQVKGKQEA
IEVYTVNEAQKYYDTLQITQLIRQTLENDK