Crystal structure of the large terminase nuclease from thermophilic phage G20c
Viral genome packaging terminase cleaves DNA using the canonical RuvC-like two-metal catalysis mechanism.
GPAMNSVFSGLDMLILLPYERRGTRLVVEDYRPDHIYCIGADFGKNQDYSVFSVLDLDTGAIACLERMNGATWSDQVARL KALSEDYGHAYVVADTWGVGDAIAEELDAQGINYTPLPVKSSSVKEQLISNLALLMEKGQVAVPNDKTILDELRNFRYYR TASGNQVMRAYGRGHDDIVMSLALAYSQYEG
0.2 M Lithium Sulfate, 0.1 M Bis-tris pH 5.5, 25% PEG3350
HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : REFINED INDIVIDUALLY
large terminase, nuclease domain, hydrolase, viral protein
NSVFSGLDMLILLPYERRGTRLVVEDYRPDHIYCIGADFGKNQDYSVFSVLDLDTGAIVCLERMNGATWSDQVARLKALS EDYGHAYVVADTWGVGDAIAEELDAQGINYTPLPVKSSSVKEQLISNLALLMEKGQVAVPNDKTILDELRNFRYYRTASG NQVMRAYGRGHDDIVMSLALAYSQYEG