Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-1,2-dideoxymannose

(2~{R},3~{S},4~{R})-2-(hydroxymethyl)oxane-3,4-diol; 1,2-dideoxy-D-mannopyranose

Contribution of Shape and Charge to the Inhibition of a Family GH99 endo-alpha-1,2-Mannanase.

MGSSHHHHHHSSGLVPRGSHMDDNNPSNSENNGGNNNLGTELDYDTFCFYYDWYGSEAIDGQYRHWAHAIAPDPNGGSGQ
NPGTIPGTQESIASNFYPQLGRYSSSDPNILTKHMDMFVMARTGVLALTWWNEQDETEAKRIGLILDAADKKKIKVCFHL
EPYPSRNVQNLRENIVKLITRYGNHPAFYRKDGKPLFFIYDSYLIEPSEWEKLLSPGGSITIRNTAYDALMIGLWTSSPT
VQRPFILNAHFDGFYTYFAATGFTYGSTPTNWVSMQKWAKENGKIFIPSVGPGYIDTRIRPWNGSVIRTRTDGQYYDAMY
RKAIEAGVSAISITSFNEWHEGSQIEPAVPYTSSEFTYLDYENREPDYYLTRTAYWVGKFRESKQ

Spanish Ministry of Economy and Competitiveness

4AD2 contains the same protein in complex with glucose-1,3-isofagomine.

4AD3 contains the same protein in complex with Glucose-1,3-deoxymannojirimycin.

4AD4 contains the same protein in complex with glucose-1,3-isofagomine and alpha-1,2- mannobiose.

4AD5 contains the same protein in complex with glucose-1,3-deoxymannojirimycin and alpha-1,2-mannobiose.

4UTF contains the same protein in complex with mannose-alpha-1,3-isofagomine and alpha- 1,2-mannobiose.

4V27 contains the same protein in complex with mannose-alpha-1,3-isofagomine.

4V28 contains an E333Q variant of the same protein with in complex in complex with Man-Man-Methylumbelliferone.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

endomannanase, endomannosidase, GH99, N-glycosylation, glycosylation, inhibitor, inhibition, shape, charge, mannobiose, mannose, glycobiology, HYDROLASE

DDNNPSNSENNGGNNNLGTELDYDTFCFYYDWYGSEAIDGQYRHWAHAIAPDPNGGSGQNPGTIPGTQESIASNFYPQLG
RYSSSDPNILTKHMDMFVMARTGVLALTWWNEQDETEAKRIGLILDAADKKKIKVCFHLEPYPSRNVQNLRENIVKLITR
YGNHPAFYRKDGKPLFFIYDSYLIEPSEWEKLLSPGGSITIRNTAYDALMIGLWTSSPTVQRPFILNAHFDGFYTYFAAT
GFTYGSTPTNWVSMQKWAKENGKIFIPSVGPGYIDTRIRPWNGSVIRTRTDGQYYDAMYRKAIEAGVSAISITSFNEWHE
GSQIEPAVPYTSSEFTYLDYENREPDYYLTRTAYWVGKFRESKQ