QTVNARKCSLTGKWTNRMNHNMTIGAVNSRGEFTGTYIATVNATSNEIKESPLHGTQNTINKRTQPTFGFTVNWKFSEST
TVFTGQCFIDRNGKEVLKTMWLLRSSVNDIGDDWKATRVGYNIFTRLRTQKEHHHHHH

Drop size: 150 nl of protein-ligand solution and well solution.
Protein-ligand solution:
1.8 mg/ml of protein solution (20 mM sodium phosphate, 1M sodium chloride, 20mM imidazole, pH 7.4) and 50 mM ligand solution (1.57 mg in 100 microL of water) were mixed in 10:1 (v/v) ratio.
Well solution:
0.18 M sodium chloride, 0.09 M sodium cacodylate (pH 6.5) and 1.8 M ammonium sulfate.

Temperature-controlled incubator was used.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Avidin, antidins, steroids, progesterone, biotin binding protein, Steroid-binding

PGLSARKCSLTGKWTNDLGSNMTIGAVNSRGEFTGTYITAVTATSNEIKESPLHGTQNTINKRTQPTFGFTVNWKFSEST
TVFTGQCFIDRNGKEVLKTMWLLRSSVNDIGDDWKATRVGINIFTRLRTQKE