Structure of plastidial phosphorylase Pho1 from Barley

Functional and structural characterization of plastidic starch phosphorylase during barley endosperm development.

MRSVASDREVRGPASTEEELSAVLTSIDSSAIASNIQHHADFTPLFSPEHSSPLKAYHATAKSVFDSLIMNWNATYDYYN
KVNAKQAYYLSMEFLQGRALTNAIGNLELTGQYAEALKQLGHNLEDVASQEPDPALGNGGLGRLASCFLDSLATLNYPAW
GYGLRYRYGLFKQIITKDGQEEVAENWLEMGNPWEIVRNDVSYPVKFYGKVVEGTDGRKHWIGGENIKAVAHDVPIPGYK
TKTTNNLRLWSTTVPSQNFDLGAFNAGDHAKANEAHLNAEKICHVLYPGDESSEGKILRLKQQYTLCSASLQDIISRFES
RAGDSLNWEDFPSKVAVQMNDTHPTLCIPELMRILMDIKGLSWNEAWSITERTVAYTNHTVLPEALEKWSLDIMQKLLPR
HVEIIETIDEELMNNIVSKYGTADISLLKQKLKDMRILDNVDLPASVAKLFIKPKEKRGKLLVESLESIAEADEKTESQE
VENILSETTEKKAESDSEEAPDAEKEDPEYELDPFAKYDPQFPRVVRMANLCVVGGHSVNGVAEIHSEIVKQDVFNSFYE
MWPTKFQNKTNGVTPRRWIRFCNPELSTIISKWIGSDDWILNTDKLAGLKKFADDEDLQSEWRTAKRNNKMKVVSLIRDK
TGYIVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMSAKDRRKSFVPRVCIFGGKAFATYVQAKRIVKFITDVAA
TVNYDPDIGDLLKVVFVPDYNVSVAETLIPASELSQHISTAGMEASGTSNMKFAMNGCLLIGTLDGANVEIREEVGEENF
FLFGAHAPEIAGLRQERAEGKFVPDLRFEEVKEYVRSGVFGTSNYDELMGSLEGNEGYGRADYFLVGKDFPSYIECQEKV
DEAYRDQKLWTRMSILNTAGSPKFSSDRTIHEYAKDIWDISPVIMPTFPDIENLYFQG

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

Starch, phosphorylase, transferase, plastidial

RSVASDREVRGPASTEEELSAVLTSIDSSAIASNIQHHADFTPLFSPEHSSPLKAYHATAKSVFDSLIMNWNATYDYYNK
VNAKQAYYLSMEFLQGRALTNAIGNLELTGQYAEALKQLGHNLEDVASQEPDPALGNGGLGRLASCFLDSLATLNYPAWG
YGLRYRYGLFKQIITKDGQEEVAENWLEMGNPWEIVRNDVSYPVKFYGKVVEGTDGRKHWIGGENIKAVAHDVPIPGYKT
KTTNNLRLWSTTVPSQNFDLGAFNAGDHAKANEAHLNAEKICHVLYPGDESSEGKILRLKQQYTLCSASLQDIISRFESR
AGDSLNWEDFPSKVAVQMNDTHPTLCIPELMRILMDIKGLSWNEAWSITERTVAYTNHTVLPEALEKWSLDIMQKLLPRH
VEIIETIDEELMNNIVSKYGTADISLLKQKLKDMRILDNVDLPASVAKLFIKPKEKRGKLLVESLESIAEADEKTESQEV
ENILSETTEKKAESDSEEAPDAEKEDPEYELDPFAKYDPQFPRVVRMANLCVVGGHSVNGVAEIHSEIVKQDVFNSFYEM
WPTKFQNKTNGVTPRRWIRFCNPELSTIISKWIGSDDWILNTDKLAGLKKFADDEDLQSEWRTAKRNNKMKVVSLIRDKT
GYIVSPDAMFDVQVKRIHEYKRQLLNILGIVYRYKKMKEMSAKDRRKSFVPRVCIFGGKAFATYVQAKRIVKFITDVAAT
VNYDPDIGDLLKVVFVPDYNVSVAETLIPASELSQHISTAGMEASGTSNMKFAMNGCLLIGTLDGANVEIREEVGEENFF
LFGAHAPEIAGLRQERAEGKFVPDLRFEEVKEYVRSGVFGTSNYDELMGSLEGNEGYGRADYFLVGKDFPSYIECQEKVD
EAYRDQKLWTRMSILNTAGSPKFSSDRTIHEYAKDIWDISPVIMP

binding site for Di-peptide PLP B 1001 and LYS B 814