Solution structure of the m-pmv myristoylated matrix protein
Membrane Interactions of the Mason-Pfizer Monkey Virus Matrix Protein and Its Budding Deficient Mutants.
The structure of myristoylated Mason-Pfizer monkey virus matrix protein and the role of phosphatidylinositol-(4,5)-bisphosphate in its membrane binding.
Myristoylated Matrix protein of Mason-Pfizer Monkey Virus. N-terminaly myristoylated with C-terminal extension with 20 AA from phospohoprotein (pp24) and his-tag.
(MYR)GQELSQHERYVEQLKQALKTRGVKVKYADLLKFFDFVKDTCPWFPQEGTIDIKRWRRVGDCFQDYYNTFGPEKVP VTAFSYWNLIKELIDKKEVNPQVMAAVAQTEEILKSNSQTDLEHHHHHH
XGQELSQHERYVEQLKQALKTRGVKVKYADLLKFFDFVKDTCPWFPQEGTIDIKRWRRVGDCFQDYYNTFGPEKVPVTAF SYWNLIKELIDKKEVNPQVMAAVAQTEEILKSNSQTDLEHHHHHH
We have assigned some aditional resonances of 2LPY structure and measured RDCs on the same protein, that were used to further improve the structure.
1.2 mM [U-99% 13C; U-99% 15N] Matrix protein p10, 100 mM potassium phosphate, 300 mM sodium chloride, 5 mM DTT, 95% H2O/5% D2O
0.2 mM [U-99% 15N] Matrix protein p10, 50 mM potassium phosphate, 150 mM sodium chloride, 5 mM DTT, 10 mg/mL Pf1 phage, 95% H2O/5% D2O
Schwieters, Kuszewski, Tjandra and Clore
Matrix, M-PMV, Myristoylated, Retrovirus, Viral protein
GQELSQHERYVEQLKQALKTRGVKVKYADLLKFFDFVKDTCPWFPQEGTIDIKRWRRVGDCFQDYYNTFGPEKVPVTAFS YWNLIKELIDKKEVNPQVMAAVAQTEEILKSNSQTDL