Crystal structure of the heterodimeric GEF Mon1-Ccz1 in complex with Ypt7

Architecture and mechanism of the late endosomal Rab7-like Ypt7 guanine nucleotide exchange factor complex Mon1-Ccz1.

PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)

GPLGSMEGFERELDNIPDTLPDDERLALWKGKLKHYLILSSAGKPIWSRHGDLSLVNSTMGVVQTIISFYEGARNPLLGF
TAGKVRFVILIKGPLYFVAISRLRESDAQLRAQLEALYMQILSTLTLPILTNIFAHRPSTDLRGPLQGTESLLASLADSF
TKGSPS

SMTTPVSPSPSGIIPAQLGFLAIYNPALGTTDETLEDQIVYYATASTLSQARRRHRRPRRRDRQRAQSVVKDSRPNAAGA
TGDSEAVAEDKDPVSKEERHERLRQIGLAQGMVEFAKSFSDGEPVDTIDTEKARVILVEVEEGWWILASIDLTRLPLPQI
KTPTSSSAPPPAPNLNPLPPEPAYEYSSREVKPPSLLRADLLRAYDLFLLHHGSSLSSLLASQGRAQLVASLTRFWDHFL
ATWNVLLHGN

GSMSSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSASYKATIGADFLTREVMVDDRQVTMQLWDTAGQERFQSLGVAFY
RGADCCVLVFDVNNAKSFDALDSWRDEFLIQASPRDPENFPFVVLGIKIDVEESKRVISTKRAQTFCQSKGGIPYFETSA
KEAINVEEAFQVIARNALMQEESEEFSGDFQDPINIHIENERDGCAC

Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

0.15 M ammonium sulfate, 0.1 M MES, pH 6.0, and 18% PEG 4000, 25% glycerol

Mon1, Ccz1, Rab small monomeric GTPase-like protein

heterodimeric GEF protein, endosomal maturation, Rab GTPase, protein transport

MEGFERELDNIPDTLPDDERLALWKGKLKHYLILSSAGKPIWSRHGDLSLVNSTMGVVQTIISFYEGARNPLLGFTAGKV
RFVILIKGPLYFVAISRLRESDAQLRAQLEALYMQILSTLTLPILTNIFAHRPSTDLRGPLQGTESLLASLADSFTKGSP
S

MTTPVSPSPSGIIPAQLGFLAIYNPALGTTDETLEDQIVYYATASTLSQARRRHRRPRRRDRQRAQSVVKDSRPNAAGAT
GDSEAVAEDKDPVSKEERHERLRQIGLAQGMVEFAKSFSDGEPVDTIDTEKARVILVEVEEGWWILASIDLTRLPLPQIK
TPTSSSAPPPAPNLNPLPPEPAYEYSSREVKPPSLLRADLLRAYDLFLLHHGSSLSSLLASQGRAQLVASLTRFWDHFLA
TWNVLLHGN

MSSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSASYKATIGADFLTREVMVDDRQVTMQLWDTAGQERFQSLGVAFYRG
ADCCVLVFDVNNAKSFDALDSWRDEFLIQASPRDPENFPFVVLGNKIDVEESKRVISTKRAQTFCQSKGGIPYFETSAKE
AINVEEAFQVIARNALMQEESEEFSGDFQDPINIHIENERDGCAC

binding site for Di-peptide SO4 F 301 and LYS F 21