Plexin A4 full extracellular region, domains 1 to 8 modeled, data to 8 angstrom, spacegroup P2(1)

Structural Basis for Plexin Activation and Regulation.

ETGKPSFVTFRGEPAEGFNHLVVDERTGHIYLGAVNRIYKLSSDLKVLVTHQTGPDEDNPKCYPPRIVQTCNEPLASTNN
VNKMLLIDYKENRLIACGSLYQGICKLLRLEDLFKLGEPFHKKEHYLSGVNESGSVFGVIVSYSNFDDKLFIATAVDGKP
EYFPTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTVIPDFDIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKE
QVYTSKLVRLCKEDTAFNSYVEVPIGCERNGVEYRLLQAAYLSKAGAVLGRTLGVRPDDDLLFTVFSKGQKRKMKSLDES
ALCIFILKQINDRIKDRLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSEMVRGIPVFTEDRDRMT
SVIAYVYKNHSLAFVGTKSGKLKKIRVDGPKGNALQYETVQVVDSGPVLRDMAFSKDHEQLYIMSERQLTRVPVESCGQY
RSCGECLGSGDPHCGWCVLHNTCTRKERCERSREPRRFASEMKQCVRLTVHPNNISVSQYNVLLVLETYNVPELSAGVNC
TFEDLSEMDGLVIGNQIQCYSPAAKEVPRIITENGDHHVVQLQLKSKETGMTFASTSFVFYNCSVHNSCLSCVESPYRCH
WCKYRHVCTHDPNTCSFQEGRVKLPEDCPQLLRVDKILVPVEVIKPITLKAKNLPQPQSGQRGYECILNIQGIEQRVPAL
RFNSSSVQCQNTSYSYEGMEINNLPVELTVVWNGHFNIDNPAQNKVYLYKCGAMRESCGLCLKADPDFECGWCQSPGQCT
LRQHCPAHESRWLELSGANSKCTNPRITEIIPVTGPREGGTKVTIRGENLGLEFRDIASHVKVAGVECSPLVDGYIPAEQ
IVCEMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLADLKPNRGPMSGGTQVTITGTNLNAGSNVVVMFGSQP
CLFHRRSPSYIICNTTSSEEVLDMKVTVQVDRARIRQDLVFQYVEDPTIVRIEPEWSIVSGNTPIAVWGTHLDLIQNPQI
RAKHGGKEHINICEVLNATEMTCQAPALALGPDHQSDLTERPEEFGFILDNVQSLLILNKTNFTYYPNPVFEAFSPSGIL
ELKPGTPIILKGKNLIPPVAGGNVKLNYTVLVGEKPCTVTVSDVQLLCESPNLIGRHKVMARVGGMEYSPGMVYIAPGRT
KHHHHHH

0.5 M sodium succinate,
100 mM bis-TRIS propane, pH 7.0

THE AUTHORS STATE THAT DUE TO THE LOW RESOLUTION OF THE DIFFRACTION DATA THE STRUCTURE WAS ONLY SUBJECTED TO RIGID BODY REFINEMENT WITH EACH DOMAIN AS RIGID BODY. THE STRUCTURE IS BASED ON A HOMOLOGY MODEL GENERATED WITH PDB ENTRY 5L56 AS TEMPLATE (53% SEQUENCE IDENTITY). THE AUTHORS HAVE NOT FURTHER REFINED THE RESULTING COORDINATES NOR CORRECTED RESULTING CLASHES BETWEEN ATOMS AND DEVIATING PEPTIDE LINKAGES BETWEEN DOMAINS.
receptor, signaling, axon guidance, signaling protein

KPSFVTFRGEPAEGFNHLVVDERTGHIYLGAVNRIYKLSSDLKVLVTHQTGPDEDNPKCYPPRIVQTCNEPLASTNNVNK
MLLIDYKENRLIACGSLYQGICKLLRLEDLFKLGEPFHKKEHYLSGVNESGSVFGVIVSYSNFDDKLFIATAVDGKPEYF
PTISSRKLTKNSEADGMFAYVFHDEFVASMIKIPSDTFTVIPDFDIYYVYGFSSGNFVYFLTLQPEMVSPPGSTTKEQVY
TSKLVRLCKEDTAFNSYVEVPIGCERNGVEYRLLQAAYLSKAGAVLGRTLGVRPDDDLLFTVFSKGQKRKMKSLDESALC
IFILKQINDRIKDRLQSCYRGEGTLDLAWLKVKDIPCSSALLTIDDNFCGLDMNAPLGVSEMVRGIPVFTEDRDRMTSVI
AYVYKNHSLAFVGTKSGKLKKIRVDGPKGNALQYETVQVVDSGPVLRDMAFSKDHEQLYIMSERQLTRVPVESCGQYRSC
GECLGSGDPHCGWCVLHNTCTRKERCERSREPRRFASEMKQCVRLTVHPNNISVSQYNVLLVLETYNVPELSAGVNCTFE
DLSEMDGLVIGNQIQCYSPAAKEVPRIITENGDHHVVQLQLKSKETGMTFASTSFVFYNCSVHNSCLSCVESPYRCHWCK
YRHVCTHDPNTCSFQEGRVKLPEDCPQLLRVDKILVPVEVIKPITLKAKNLPQPQSGQRGYECILNIQGIEQRVPALRFN
SSSVQCQNTSYSYEGMEINNLPVELTVVWNGHFNIDNPAQNKVYLYKCGAMRESCGLCLKADPDFECGWCQSPGQCTLRQ
HCPAHESRWLELSGANSKCTNPRITEIIPVTGPREGGTKVTIRGENLGLEFRDIASHVKVAGVECSPLVDGYIPAEQIVC
EMGEAKPSQHAGFVEICVAVCRPEFMARSSQLYYFMTLTLADLKPNRGPMSGGTQVTITGTNLNAGSNVVVMFGSQPCLF
HRRSPSYIICNTTSSEEVLDMKVTVQVDRARIRQDLVFQYVEDPTIVRIEPEWSIVSGNTPIAVWGTHLDLIQNPQIRAK
HGGKEHINICEVLNATEMTCQAPALALGPDHQSDLTERPEEFGFILDNVQSLLILNKTNFTYYPNPVFEAFSPSGILELK
PGTPIILKGKNLIPPVAGGNVKLNYTVLVGEKPCTVTVSDVQLLCESPNLIGRHKVMARVGGMEYSPGMVYIAP