Crystal structure of Tryptophan synthase alpha-beta chain complex from Francisella tularensis

Center for Structural Genomics of Infectious Diseases (CSGID)

(2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid

Conservation of the structure and function of bacterial tryptophan synthases.

SNA(MSE)TNRYTTLFANLEKRNEGAFIPFVTIGDPNKALSFEIIDTLVSSGADALELGIPFSDPLADGPTIQEANIRAL
ESGITPKDCFDILTKIRAKYPHIPIGLLLYANLVYANGIENFYQKCLDAGVDSILIADVPAHESKEFRDIAKKVGIAQIF
IAPPDASESTLKQISELGSGYTYLLSRVGVTGTETAAN(MSE)PVEDVLTKLREYNAPKPVLGFGISKPEQVQQAIKAGA
AGAISGSATVKIIQNNISNKQK(MSE)LNELTYFVKE(MSE)KAATLN

SNAMTNRYTTLFANLEKRNEGAFIPFVTIGDPNKALSFEIIDTLVSSGADALELGIPFSDPLADGPTIQEANIRALESGI
TPKDCFDILTKIRAKYPHIPIGLLLYANLVYANGIENFYQKCLDAGVDSILIADVPAHESKEFRDIAKKVGIAQIFIAPP
DASESTLKQISELGSGYTYLLSRVGVTGTETAANMPVEDVLTKLREYNAPKPVLGFGISKPEQVQQAIKAGAAGAISGSA
TVKIIQNNISNKQKMLNELTYFVKEMKAATLN

(MSE)SKLNAYFGEYGGQFVPQILVPALDQLEQEFIKAQADESFKQEFKELLQEYAGRPTALTKTRNIVKNTRTKLYLKR
EDLLHGGAH(LLP)TNQVLGQALLAKR(MSE)GKKEIIAETGAGQHGVATALACALLDLKCRVY(MSE)GAKDVERQSPN
VFR(MSE)KL(MSE)GAEVIPVHSGSATLKDACNEALRDWSANYSKAHYLLGTAAGPHPFPTIVREFQR(MSE)IGEETK
QQ(MSE)LAKEGRLPDAVIACVGGGSNAIG(MSE)FADFIDEKNVKLIGVEPAGKGIETGEHGAPLKHGKTGIFFG
(MSE)KAPL(MSE)QNSDGQIEESYSISAGLDFPSVGPQHAHLLAIGRAKYASATDDEALDAFKLLCKKEGIIPALESSH
ALAHALKLAYEDPNKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

MSKLNAYFGEYGGQFVPQILVPALDQLEQEFIKAQADESFKQEFKELLQEYAGRPTALTKTRNIVKNTRTKLYLKREDLL
HGGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATALACALLDLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHS
GSATLKDACNEALRDWSANYSKAHYLLGTAAGPHPFPTIVREFQRMIGEETKQQMLAKEGRLPDAVIACVGGGSNAIGMF
ADFIDEKNVKLIGVEPAGKGIETGEHGAPLKHGKTGIFFGMKAPLMQNSDGQIEESYSISAGLDFPSVGPQHAHLLAIGR
AKYASATDDEALDAFKLLCKKEGIIPALESSHALAHALKLAYEDPNKEQLLVVNLSGRGDKDIFTVHDILKEKGEI

Francisella tularensis subsp. tularensis

Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)

0.2M calcium acetate, 0.1M imidazole, 10% PEG 8000

Center for Structural Genomics of Infectious Diseases
NIAID, National Institute of Allergy and Infectious Diseases

Tryptophan synthase alpha chain (E.C.4.2.1.20), Tryptophan synthase beta chain (E.C.4.2.1.20)

Structural Genomics, PSI-Biology, Midwest Center for Structural Genomics, MCSG, Center for Structural Genomics of Infectious Diseases, CSGID, LYASE

MTNRYTTLFANLEKRNEGAFIPFVTIGDPNKALSFEIIDTLVSSGADALELGIPFSDPLADGPTIQEANIRALESGITPK
DCFDILTKIRAKYPHIPIGLLLYANLVYANGIENFYQKCLDAGVDSILIADVPAHESKEFRDIAKKVGIAQIFIAPPDAS
ESTLKQISELGSGYTYLLSRVGVTGTETAANMPVEDVLTKLREYNAPKPVLGFGISKPEQVQQAIKAGAAGAISGSATVK
IIQNNISNKQKMLNELTYFVKEMKAATLN

SKLNAYFGEYGGQFVPQILVPALDQLEQEFIKAQADESFKQEFKELLQEYAGRPTALTKTRNIVKNTRTKLYLKREDLLH
GGAHKTNQVLGQALLAKRMGKKEIIAETGAGQHGVATALACALLDLKCRVYMGAKDVERQSPNVFRMKLMGAEVIPVHSG
SATLKDACNEALRDWSANYSKAHYLLGTAAGPHPFPTIVREFQRMIGEETKQQMLAKEGRLPDAVIACVGGGSNAIGMFA
DFIDEKNVKLIGVEPAGKGIETGEHGAPLKHGKTGIFFGMKAPLMQNSDGQIEESYSISAGLDFPSVGPQHAHLLAIGRA
KYASATDDEALDAFKLLCKKEGIIPALESSHALAHALKLAYEDPNKEQLLVVNLSGRGDKDIFTVHDILKEKGEI