CRYSTAL STRUCTURE OF THE TWO TANDEM RRM DOMAINS OF PUF60 BOUND TO A PORTION OF AN ADML PRE-MRNA 3' SPLICE SITE ANALOG

Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.

Dimerization of FIR upon FUSE DNA binding suggests a mechanism of c-myc inhibition

analog of 3' intron sequence of the adenovirus major late promoter

60 kDa poly(U)-binding-splicing factor,FUSE-binding protein-interacting repressor,FBP-interacting repressor,Ro-binding protein 1,RoBP1,Siah-binding protein 1,Siah-BP1

GSHMASMTGGQQMGRGSAAQRQGALAIMSRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVP
EAAQLALEQMNSVMLGGRNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSATLARD
PTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPG

(DG)(DA)(DU)(DG)(DU)(DC)(DA)(DU)(DA)(DC)(DU)(DU)(DA)(DU)(DC)(DC)(DU)(DG)(DU)(DC)
(DC)(DC)(DU)(DU)(DU)(DU)(DU)(DU)(DU)(DU)

0.1 M TRIS-HCl, 25% PEG 4000, 5-10 mM Barium Chloride Dihydrate, pH 8.7, mixed with 10 mg/ml protein-nucleic acid mixture in 50 mM TRIS-HCl, pH 8.0, 150 mM NaCl, 20 micromolar EDTA.

Sequence from the adenovirus major late pre-mRNA 3'splice site

DATA WERE DE-TWINNED DURING REFINEMENT
PROCESS

Authors have supported the biological unit analysis by performing size exclusion chromatography and light scattering

TANDEM RRMS, PROTEIN-NUCLEIC ACID COMPLEX, SPLICING FACTOR, SPLICING-DNA complex

AAQRQRALAIMCRVYVGSIYYELGEDTIRQAFAPFGPIKSIDMSWDSVTMKHKGFAFVEYEVPEAAQLALEQMNSVMLGG
RNIKVGRPSNIGQAQPIIDQLAEEARAFNRIYVASVHQDLSDDDIKSVFEAFGKIKSCTLARDPTTGKHKGYGFIEYEKA
QSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPG