Human Islet Amyloid Polypeptide Segment 19-SGNNFGAILSS-29 with Early Onset S20G Mutation Determined by MicroED
Atomic structures of fibrillar segments of hIAPP suggest tightly mated beta-sheets are important for cytotoxicity.
1 mM lyophilized peptide in PBS with 1% DMSO at room temperature under quiescent conditions. Crystals grew in a few hours and reached full size within 15 hours.
Phasing statistics are not applicable. No imaging was used. The phases were obtained using molecular replacement.
The detector was operated in rolling shutter mode with 2x2 pixel binning.
GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER
Amylin, Diabetes-associated peptide, DAP, Insulinoma amyloid peptide
1 mmol SGNNFGAILSS in 1 L phosphate-buffered saline with 1% DMSO incubated under quiescent conditions overnight
National Institutes of Health/National Institute on Aging (NIH/NIA)
The biological unit is an extended pair of beta sheets comprising peptides at positions X,Y,Z and 1/2+X,-1/2-Y,-Z repeated ad infinitum along the a crystal axis.
Amyloid, islet amyloid polypeptide, Type II Diabetes, Toxic Spine, MicroED, PROTEIN FIBRIL