L16A mutant of cytochrome c prime from Alcaligenes xylosoxidans: Ferrous state

Engineering proximal vs. distal heme-NO coordination via dinitrosyl dynamics: implications for NO sensor design.

(PCA)FAKPEDAVKYRQSAATLMASHFGRMTPVVKGQAPYDAAQIKANVEVLKTLSALPWAAFGPGTEGGDARPEIWSDA
ASFKQKQQAFQDNIVKLSAAADAGDLDKLRAAFGDVGASCKACHDAYRK

QFAKPEDAVKYRQSAATLMASHFGRMTPVVKGQAPYDAAQIKANVEVLKTLSALPWAAFGPGTEGGDARPEIWSDAASFK
QKQQAFQDNIVKLSAAADAGDLDKLRAAFGDVGASCKACHDAYRK

2.2M Ammonium sulfate
0.1 M HEPES pH 7.5

ANISOTROPIC REFINEMENT REDUCED FREE R (NO CUTOFF) BY ?

gas sensor, cytochrome, nitric oxide, electron transport

QFAKPEDAVKYRQSALTLMASHFGRMTPVVKGQAPYDAAQIKANVEVLKTLSALPWAAFGPGTEGGDARPEIWSDAASFK
QKQQAFQDNIVKLSAAADAGDLDKLRAAFGDVGASCKACHDAYRK