Structure of an oxidoreductase SeMet-labelled from Synechocystis sp. PCC6803

Unprecedented pathway of reducing equivalents in a diflavin-linked disulfide oxidoreductase.

Pyridine nucleotide-disulfide oxidoreductase

GSH(MSE)VKLSSKNLDARLDTVYDAIVLGGG(MSE)GGLSAAIYLARYGLKCLVVEKGRGRSFW(MSE)QDLRNYVGLD
PDTPGRDIITHSTQQALHWGADLLRGYVEDVTDEGDTLAVKVKVGKKDSLYPIFRTKYVIAATGIIDNLPQLED(MSE)Q
NVYDYAGYTLHVC(MSE)ICDGFD(MSE)WDQKAVLIAGTEGQINAAFVLNWFTPYITVLTHGLCTVGDE(MSE)KAKLA
DHGYPLHEAAITKFLGEDHK(MSE)SGVELVDGTV(MSE)EATTGLIN(MSE)GSVYHNHYLKGIEGLEWDGENLVTND
(MSE)AQTSHPRIFALGDLKKGLNQVSVAVADGTLAATQIWRNIRRASEPRKWIH

GSHMVKLSSKNLDARLDTVYDAIVLGGGMGGLSAAIYLARYGLKCLVVEKGRGRSFWMQDLRNYVGLDPDTPGRDIITHS
TQQALHWGADLLRGYVEDVTDEGDTLAVKVKVGKKDSLYPIFRTKYVIAATGIIDNLPQLEDMQNVYDYAGYTLHVCMIC
DGFDMWDQKAVLIAGTEGQINAAFVLNWFTPYITVLTHGLCTVGDEMKAKLADHGYPLHEAAITKFLGEDHKMSGVELVD
GTVMEATTGLINMGSVYHNHYLKGIEGLEWDGENLVTNDMAQTSHPRIFALGDLKKGLNQVSVAVADGTLAATQIWRNIR
RASEPRKWIH

100mM Bis-tris propane, pH 7.5
200 mM Na2SO4
20% PEG 3350

KLSSKNLDARLDTVYDAIVLGGGMGGLSAAIYLARYGLKCLVVEKGRGRSFWMQDLRNYVGLDPDTPGRDIITHSTQQAL
HWGADLLRGYVEDVTDEGDTLAVKVKVGKKDSLYPIFRTKYVIAATGIIDNLPQLEDMQNVYDYAGYTLHVCMICDGFDM
WDQKAVLIAGTEGQINAAFVLNWFTPYITVLTHGLCTVGDEMKAKLADHGYPLHEAAITKFLGEDHKMSGVELVDGTVVE
ATTGLINMGSVYHNHYLKGIEGLEWDGENLVTNDMAQTSHPRIFALGDLKKGLNQVSVAVADGTLAATQIWRNIRRASEP
RKWIH