Macrolide 2'-phosphotransferase type II - complex with GDP and erythromycin

Structural Basis for Kinase-Mediated Macrolide Antibiotic Resistance.

Macrolide 2'-phosphotransferase II protein MphB,Macrolide 2-phosphotransferase,mph(B)

MSKDIKQVIEIAKKHNLFLKEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAP
NWIIYTEELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMK
QRMDAVRAKFGVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAFG
EEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAKEALEVQGS

0.2 M calcium acetate, 0.1M Tris, 25-40% PEG 4000

Canadian Institutes of Health Research (CIHR)

macrolide phosphotransferase, kinase, TRANSFERASE