Crystal structure of E. coli Cascade bound to a PAM-containing dsDNA target (32-nt spacer) at 3.20 angstrom resolution.

GUANOSINE-5'-PHOSPHATE-2',3'-CYCLIC PHOSPHATE

Structural basis for promiscuous PAM recognition in type I-E Cascade from E. coli.

CRISPR type I-E/Ecoli-associated protein CasA/Cse1,CRISPR-associated protein CasA/Cse1

MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEF
QQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGG
GFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIE
LCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQ
NENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFA
EGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLA
LARATLYKHLRELKPQGGPSNG

MASHHHHHHGALEVLFQGPGYQMADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQAL
LRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLT
WWGKRERQQLLEDFVLTTNKNA

MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLG
ERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDI
ALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLG
GASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVA
NGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA

MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVT
GLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLG
TCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ

(DG)(DT)(DG)(DC)(DA)(DG)(DT)(DG)(DC)(DT)(DC)(DG)(DA)(DT)(DG)(DT)(DT)(DT)(DT)(DA)
(DT)(DT)(DT)(DA)(DT)(DT)(DT)(DT)(DA)(DT)(DT)(DT)(DA)(DT)(DT)(DA)(DT)(DT)(DT)(DA)
(DT)(DT)(DT)(DT)(DA)(DT)(DA)

(DC)(DT)(DG)(DT)(DT)(DG)(DG)(DC)(DA)(DA)(DG)(DC)(DC)(DA)(DG)(DG)(DA)(DT)(DC)(DT)
(DG)(DA)(DA)(DC)(DA)(DA)(DT)(DA)(DC)(DC)(DG)(DT)(DC)(DA)(DT)(DC)(DG)(DA)(DG)(DC)
(DA)(DC)(DT)(DG)(DC)(DA)(DC)

MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQV
GVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQT
VCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL

CRISPR Cascade, Immune System-RNA Complex

MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSE
QTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA

binding site for Di-nucleotide G L 60 and 23G L 61