Farnesylated RhoA-GDP in complex with RhoGDI-alpha, lysine acetylated at K178

Structural and Mechanistic Insights Into the Regulation of the Fundamental Rho-Regulator Rhogdi Alpha by Lysine Acetylation.

THIOESTER BETWEEN CYS190 AND FARNESYL-MOIETY

GSMAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVADIEVDGKQVELALWDTAGQEDYDRLRPLSYPDTD
VILMCFSIDSPDSLENIPEKWTPEVKHFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYME
CSAKTKDGVREVFEMATRAALQARRGKKKSGCLVL

HMAHHHHHHMAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVV
VTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGP
RAEEYEFLTPMEEAPKGMLARGSYNI(ALY)SRFTDDDRTDHLSWEWNLTIKKEWKD

HMAHHHHHHMAEQEPTAEQLAQIAAENEEDEHSVNYKPPAQKSIQEIQELDKDDESLRKYKEALLGRVAVSADPNVPNVV
VTRLTLVCSTAPGPLELDLTGDLESFKKQSFVLKEGVEYRIKISFRVNREIVSGMKYIQHTYRKGVKIDKTDYMVGSYGP
RAEEYEFLTPMEEAPKGMLARGSYNIKSRFTDDDRTDHLSWEWNLTIKKEWKD

0.2 M LITHIUMSULFATE 0.1 M BIS-TRIS PH5.5 25% PEG3350 30% DEXTROSE

DOUBLE ACETYLATED RHOGDI-ALPHA IN COMPLEX WITH RHOA- GDP

SIGNALING PROTEIN, LYSINE-ACETYLATION, RHOA, RAS-SUPERFAMILY, RHOGDI, CYTOSKELETON, GDP