Crystal structure of NKX2-5 and TBX5 bound to the Nppa promoter region

Complex Interdependence Regulates Heterotypic Transcription Factor Distribution and Coordinates Cardiogenesis.

HOMEOBOX PROTEIN NKX-2.5, T-BOX TRANSCRIPTION FACTOR TBX5
DNA BINDING DOMAINS OF TBX5 AND NKX2-5, UNP RESIDUES 134-197 AND 51-251

5'-D(*TP*CP*TP*TP*CP*TP*CP*AP*CP*AP*CP*CP*TP*TP *TP*GP*AP*AP*GP*TP*GP*G)-3'

5'-D(*AP*CP*CP*AP*CP*TP*TP*CP*AP*AP*AP*GP*GP*TP *GP*TP*GP*AP*GP*AP*AP*G)-3'

CARDIAC-SPECIFIC HOMEOBOX, HOMEOBOX PROTEIN CSX, HOMEOBOX PROTEIN NK-2 HOMOLOG E, T-BOX PROTEIN 5

GAMGARRRRKPRVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKSKRQRQSSSSSSSSSSSS
SSSAMEGIKVFLHERELWLKFHEVGTEMIITKAGRRMFPSYKVKVTGLNPKTKYILLMDIVPADDHRYKFADNKWSVTGK
AEPAMPGRLYVHPDSPATGAHWMRQLVSFQKLKLTNNHLDPFGHIILNSMHKYQPRLHIVKADENNGFGSKNTAFSTHVF
PETAFIAVTSYQNHKITQLKIENNPFAKGFRGSDDLELHRMSRMQ

(DT)(DC)(DT)(DT)(DC)(DT)(DC)(DA)(DC)(DA)(DC)(DC)(DT)(DT)(DT)(DG)(DA)(DA)(DG)(DT)
(DG)(DG)

(DA)(DC)(DC)(DA)(DC)(DT)(DT)(DC)(DA)(DA)(DA)(DG)(DG)(DT)(DG)(DT)(DG)(DA)(DG)(DA)
(DA)(DG)

0.1M HEPES PH7.5 0.1 M KBR, 0.2M NA-ACETATE AND 20 % PEG 3000

DOUBLE-HETEROHEXAMERIC RINGS OF FULL-LENGTH RVB1(ADP) RVB2(APO)

DOUBLE-HETEROHEXAMERIC RINGS OF FULL-LENGTH RVB1(ADP) RVB2(ADP)

PROTEIN FUSION OF MNKX2-5(AA134-197,UNIPROT-P42582)AND MTBX5(AA51-251, UNIPROT-P70326) USING A 15SER LINKER AND ALL CYSTEINE RESIDUES MUTATED TO SERINE RESIDUES MUTATION CYS TO SER

CHAIN 'A' AND (RESID 1053 THROUGH 1075 )

CHAIN 'A' AND (RESID 1076 THROUGH 1088 )

CHAIN 'A' AND (RESID 1089 THROUGH 1113 )

CHAIN 'A' AND (RESID 1114 THROUGH 1123 )

CHAIN 'A' AND (RESID 1124 THROUGH 1134 )

CHAIN 'A' AND (RESID 1135 THROUGH 1187 )

CHAIN 'A' AND (RESID 1188 THROUGH 1214 )

CHAIN 'A' AND (RESID 1215 THROUGH 1227 )

CHAIN 'A' AND (RESID 1228 THROUGH 1238 )

CHAIN 'E' AND (RESID 1054 THROUGH 1075 )

CHAIN 'E' AND (RESID 1076 THROUGH 1108 )

CHAIN 'E' AND (RESID 1109 THROUGH 1120 )

CHAIN 'E' AND (RESID 1121 THROUGH 1150 )

CHAIN 'E' AND (RESID 1151 THROUGH 1160 )

CHAIN 'E' AND (RESID 1161 THROUGH 1187 )

CHAIN 'E' AND (RESID 1188 THROUGH 1214 )

CHAIN 'E' AND (RESID 1215 THROUGH 1227 )

CHAIN 'E' AND (RESID 1228 THROUGH 1237 )

CHAIN 'I' AND (RESID 1056 THROUGH 1068 )

CHAIN 'I' AND (RESID 1069 THROUGH 1085 )

CHAIN 'I' AND (RESID 1086 THROUGH 1116 )

CHAIN 'I' AND (RESID 1117 THROUGH 1136 )

CHAIN 'I' AND (RESID 1137 THROUGH 1148 )

CHAIN 'I' AND (RESID 1149 THROUGH 1158 )

CHAIN 'I' AND (RESID 1159 THROUGH 1187 )

CHAIN 'I' AND (RESID 1188 THROUGH 1227 )

CHAIN 'I' AND (RESID 1228 THROUGH 1237 )

CHAIN 'M' AND (RESID 1053 THROUGH 1075 )

CHAIN 'M' AND (RESID 1076 THROUGH 1088 )

CHAIN 'M' AND (RESID 1089 THROUGH 1100 )

CHAIN 'M' AND (RESID 1101 THROUGH 1126 )

CHAIN 'M' AND (RESID 1127 THROUGH 1150 )

CHAIN 'M' AND (RESID 1151 THROUGH 1160 )

CHAIN 'M' AND (RESID 1161 THROUGH 1186 )

CHAIN 'M' AND (RESID 1187 THROUGH 1209 )

CHAIN 'M' AND (RESID 1210 THROUGH 1237 )