Structure of the in vitro assembled bacteriophage phi6 polymerase complex

Localized Reconstruction of Subunits from Electron Cryomicroscopy Images of Macromolecular Complexes.

METHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY

SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3185. (DEPOSITION ID: 13852).

BACTERIOPHAGE PHI6 POLYMERASE COMPLEX ASSEMBLED IN VITRO FROM PURIFIED PROTEINS P1, P2, AND P4

VITRIFICATION 1 -- CRYOGEN- ETHANE, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV, METHOD- BLOT 4 SECONDS BEFORE PLUNGING,

GFNLKVKDLNGSARGLTQAFAIGELKNQLSVGALQLPLQFTRTFSASMTSELLWEVGKGNIDPVMYARLFFQYAQAGGAL
SVDELVNQFTEYHQSTACNPEIWRKLTAYITGSSNRAIKADAVGKVPPTAILEQLRTLAPSEHELFHHITTDFVCHVLSP
LGFILPDAAYVYRVGRTATYPNFYALVDCVRASDLRRMLTALSSVDSKMLQATFKAKGALAPALISQHLANAATTAFERS
RGNFDANAVVSSVLTILGRLWSPSTPKELDPSARLRNTNGIDQLRSNLALFIAYQDMVKQRGRAEVIFSDEELSSTIIPW
FIEAMSEVSPFKLRPINETTSYIGQTSAIDHMGQPSHVVVYEDWQFAKEITAFTPVKLANNSNQRFLDVEPGISDRMSAT
LAPIGNTFAVSAFVKNRTAVYEAVSQRGTVNSNGAEMTLGFPSVVERDYALDRDPMVAIAALRTGIVDESLEARASNDLK
RSMFNYYAAVMHYAVAHNPEVVVSEHQGVAAEQGSLYLVWNVRTELRIPVGYNAIEGGSIRTPEPLEAIAYNKPIQPSEV
LQAKVLDLANHTTSIHIWPWHEASTEFAYEDAYSVTIRNKRYTAEVKEFELLGLGQRRERVRILKPTVAHAIIQMWYSWF
VEDDRTLAAARRTSRDDAEKLAIDGRRMQNAVTLLRKIEMIGTTGIGASAVHLAQSRIVDQMAGRGLIDDSSDLHVGINR
HRIRIWAGLAVLQMMGLLSRSEAEALTKVLGDSNALGMVVA

STRUCTURE OF THE P2 POLYMERASE INSIDE IN VITRO ASSEMBLED BACTERIOPHAGE PHI6 POLYMERASE COMPLEX

STRUCTURE OF THE P2 POLYMERASE INSIDE IN VITRO ASSEMBLED BACTERIOPHAGE PHI6 POLYMERASE COMPLEX, WITH P1 INCLUDED

icosahedral asymmetric unit, std point frame