E.coli GlpG Y205F mutant complexed with aldehyde inhibitor in DMPC/CHAPSO bicelle
Crystal Structures and Inhibition Kinetics Reveal a Two-Stage Catalytic Mechanism with Drug Design Implications for Rhomboid Proteolysis.
Acetylation at N-terminus and aldehyde group at C-terminus
peptidic derivative of Gurken: ACE-VAL-ARG-MET-ALA-aldehyde
MGSSHHHHHHSSGLVPRGSHMAALRERAGPVTWVMMIACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMH FSLMHILFNLLWWWYLGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVFALMGYVWLRGERDPQSGIYL QRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
3M NaCl, 0.1M Sodium acetate pH 4.5, 5% Glycerol, 7% DMPC/CHAPSO bicelle
Rhomboid protease GlpG (E.C.3.4.21.105), peptidic derivative of Gurken
Glpg, Rhomboid, bicelle, aldehyde inhibitor, HYDROLASE-INHIBITOR complex, HYDROLASE-HYDROLASE INHIBITOR complex
AALRERAGPVTWVMMIACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWYLGGAVE KRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERDPQSGIYLQRGLIIFALIWIVAGWFDLFG MSMANGAHIAGLAVGLAMAFVDSLNARKRK