Crystal structure of the epimerase SnoN in complex with Ni2+, succinate and nogalamycin RO

Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway.

MAHHHHHHHRSADQETEPGVPADLPAESDPAALERLAARYRRDGYVHVPGVLDAGEVAEYLAEARRLLAHEESVRWGSGA
GTVMDYVADAQLGSDTMRRLATHPRIAALAEYLAGSPLRLFKLEVLLKENKEKDASVPTAPHHDAFAFPFSTAGTALTAW
VALVDVPVERGCMTFVPGSHLLPDPDTGDEPWAGAFTRPGEIWMPRVTVPLRAGDCTFHHARTVHSAGANSTDEPRLSTS
AVYMDATAAYRPTGIAFLDDLPGTGADPLREGAPLTGDRFPLLRRPQTRQPAPARYPLPQQGHGS

PEG3350, Bis-Tris, MgCl2, NiCl2, sodium succinate, nogalamycin RO

this entry contains the same protein complexed with Ni2+, succinate and nogalamycin RO

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS

alpha ketoglutarate dependent epimerase, substrate complex, nogalamycin biosynthesis, isomerase

QETEPGVPADLPAESDPAALERLAARYRRDGYVHVPGVLDAGEVAEYLAEARRLLAHEESVRWGSGAGTVMDYVADAQLG
SDTMRRLATHPRIAALAEYLAGSPLRLFKLEVLLKENKEKDASVPTAPHHDAFAFPFSTAGTALTAWVALVDVPVERGCM
TFVPGSHLLPDPDTGDEPWAGAFTRPGEI

WMPRVTVPLRAGDCTFHHARTVHSAGANSTDEPRLSTSAVYMDATAAYRPTGIAFLDDLPGTGADPLREGAPLTGDRFPL
LRRPQTRQPAPARYPLPQQGHGS