Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with splayed ends

Foreign DNA capture during CRISPR-Cas adaptive immunity.

SMTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVG
EAGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVT
WNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRN
PGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDAGHRSS

MMSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRT
PVDLDGLRLVSFLPVGSSENLYFQ

(DC)(DA)(DT)(DC)(DT)(DA)(DA)(DA)(DC)(DA)(DC)(DC)(DA)(DG)(DA)(DA)(DC)(DG)(DA)(DG)
(DT)(DA)(DG)(DT)(DA)(DA)(DA)(DT)(DT)(DG)(DG)(DG)(DC)

(DT)(DA)(DA)(DA)(DC)(DA)(DT)(DT)(DT)(DA)(DC)(DT)(DA)(DC)(DT)(DC)(DG)(DT)(DT)(DC)
(DT)(DG)(DG)(DT)(DG)(DT)(DT)(DT)(DC)(DT)(DC)(DG)(DT)

100 mM sodium citrate tribasic pH 5.6, 200 mM sodium acetate and 8% PEG 8000 (w/v)

4P6I contains the same protein complex in Apo form.

Adaptive Immunity, CRISPR-Associated Proteins, CRISPR-Cas Systems, Clustered Regularly Interspaced Short Palindromic Repeats, Integrases, Endodeoxyribonucleases, Endonucleases, DNA binding protein, Hydrolase-DNA complex

MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGE
AGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
NGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNP
GEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDAGHRSS

MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTP
VDLDGLRLVSFLPV