Crystal Structure of Cas-DNA-PAM complex

Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems.

MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGE
AGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTW
NGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNP
GEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDAGHRSS

(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DA)(DG)(DC)(DT)(DG)(DA)(DG)(DG)(DG)(DC)(DC)(DT)
(DC)(DA)(DG)(DC)(DT)(DA)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DT)(DT)(DT)

(DT)(DT)(DT)(DT)(DT)(DC)(DG)(DT)(DA)(DG)(DC)(DT)(DG)(DA)(DG)(DG)(DC)(DC)(DC)(DT)
(DC)(DA)(DG)(DC)(DT)(DA)(DC)(DG)(DT)(DT)(DT)(DT)(DC)(DT)(DT)(DT)

MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTP
VDLDGLRLVSFLPV

50mM Na cacodylate,0.9mM spermine,18mM Magnesium chloride, 1.8mM Cobalt chloride, 9% isopropanol

protein-DNA complex, HYDROLASE-DNA complex