R2-like ligand-binding oxidase with aerobically reconstituted Mn/Fe cofactor (short soak)

Structural Basis for Oxygen Activation at a Heterodinuclear Manganese/Iron Cofactor.

MAHHHHHHVDDDDKMVHHDGFQTVKATIDWEHPMFKLYEKAKRNGKWNPADIDFSQDQKDFASLTSEEKISALPLVAGFS
AGEEAVTLDILPMAHALARQGRLEDVLFLTTFMHDEAKHVEMFSRWQQAVGIGQMDLSVFHNDHYKRIFYEALPEAMNRL
YADDSPEAVIRAATVYNMIVEGTLAESGYYTFRQIYKKAGLFPGLLQGIDYLNMDEGRHIQFGIYTIQRIVNEDERYYEL
FIRYMDELWPHVIGYVDYLTELGKRQQQLARTYALEIDYDLLRHYVIKQFNLRKKQISRTKRVDVVEGLEKTAAES

Geobacillus kaustophilus (strain HTA426)

4HR0 contains the same protein with aerobically reconstituted Mn/Fe cofactor (short soak, protein purified in partially metal-bound form).

4HR4 contains the same protein with anaerobically reconstituted Mn/Fe cofactor.

4HR5 contains the same protein without metal cofactor.

4XBV contains the same protein with anaerobically reconstituted diiron cofactor.

4XB9 contains the same protein with aerobically reconstituted diiron cofactor (long soak).

5DCO contains the same protein with aerobically reconstituted diiron cofactor (short soak).

4XBW contains the same protein with aerobically reconstituted dimanganese cofactor.

R2-LIKE LIGAND-BINDING OXIDASE, DIIRON COFACTOR, RIBONUCLEOTIDE REDUCTASE R2 SUBUNIT FOLD, METALLOPROTEIN OXIDOREDUCTASE, OXIDOREDUCTASE

MVHHDGFQTVKATIDWEHPMFKLYEKAKRNGKWNPADIDFSQDQKDFASLTSEEKISALPLVAGFSAGEEAVTLDILPMA
HALARQGRLEDVLFLTTFMHDEAKHVEMFSRWQQAVGIGQMDLSVFHNDHYKRIFYEALPEAMNRLYADDSPEAVIRAAT
VYNMIVEGTLAESGYYTFRQIYKKAGLFPGLLQGIDYLNMDEGRHIQFGIYTIQRIVNEDERYYELFIRYMDELWPHVIG
YVDYLTELGKRQQQLARTYALEIDYDLLRHYVIKQFNLRKKQISRTKRVDVVEGLEKTAAES