Luciferin-regenerating enzyme solved by SIRAS using XFEL (refined against Hg derivative data)

An isomorphous replacement method for efficient de novo phasing for serial femtosecond crystallography.

GSHMGPVVEKIAELGKYTVGEGPHWDHETQTLYFVDTVEKTFHKYVPSQKKYTFCKVDKLVSFIIPLAGSPGRFVVSLER
EIAILTWDGVSAAPTSIEAIVNVEPHIKNNRLNDGKADPLGNLWTGTMAIDAGLPIGPVTGSLYHLGADKKVKMHESNIA
IANGLAWSNDLKKMYYIDSGKRRVDEYDYDASTLSISNQRPLFTFEKHEVPGYPDGQTIDEEGNLWVAVFQGQRIIKIST
QQPEVLLDTVKIPDPQVTSVAFGGPNLDELYVTSAGLQLDDSSFDKSLVNGHVYRVTGLGVKGFAGVKVKL

mixing purified LRE solution (27 mg/mL LRE, 10 mM HEPES pH 7.5, 0.1 M NaCl, 10% glycerol) and precipitant solution (35% PEG3350, 10% MPD, 0.1 M HEPES pH 7.5, 0.2 M MgCl2) at ratios between 1:2 and 1:1.6

The depositors believe that residues ILE 133, TYR 268, PHE 281 are correct and that UniProt is incorrect at these positions

MGPVVEKIAELGKYTVGEGPHWDHETQTLYFVDTVEKTFHKYVPSQKKYTFCKVDKLVSFIIPLAGSPGRFVVSLEREIA
ILTWDGVSAAPTSIEAIVNVEPHIKNNRLNDGKADPLGNLWTGTMAIDAGLPVGPVTGSLYHLGADKKVKMHESNIAIAN
GLAWSNDLKKMYYIDSGKRRVDEYDYDASTLSISNQRPLFTFEKHEVPGYPDGQTIDEEGNLWVAVFQGQRIIKISTQQP
EVLLDTVKIPDPQVTSVAFGGPNLDELHVTSAGLQLDDSSLDKSLVNGHVYRVTGLGVKGFAGVKVKL