PreQ1-II riboswitch with an engineered G-U wobble pair bound to Cs+

Cesium(I) binding to G-U-wobble base pairs in preQ1 riboswitches with implications for crystallographic phasing

Si(111) Side scattering bent cube-root I-beam single crystal; asymmetric cut 4.965 degs

(GTP)GAAGGCCGAAAGGUCUUCCACGACGAUACUUAUUUCCUUUGAUCGUCGUUAUUACUGGCUUCGGCCACAAAGGAG
A

Thin plates of size 300 um x 50 um x 20 um

14.4% (w/v) poly(ethylene) glycol 6000, 0.130 M MgOAc2, 0.001 M spermine-HCl, 0.020 M KCl, 0.130 M CsCl and 0.050 M Na-cacodylate pH 6.0

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

National Institutes of Health/National Center for Research Resources (NIH/NCRR)

4jf2 does not possess the engineered G-U wobble pair of the new entry and crystallized with a single molecule in the asymmetric unit rather than four molecules.

This sequence was obtained from the 5-prime-leader of the queT gene

GU wobble, pseudoknot, Shine-Dalgarno sequence, RNA