Crystal structure of E. coli YaaA, a member of the DUF328/UPF0246 family

The DUF328 family member YaaA is a DNA-binding protein with a novel fold.

(MSE)GSSHHHHHHSSGLVPRGSH(MSE)LILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPPQISTL
(MSE)RISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFDFAQQHLR(MSE)LSGLYGVLRPL
DL(MSE)QPYRLE(MSE)GIRLENARGKDLYQFWGDIITNKLNEALAAQGDNVVINLASDEYFKSVKPKKLNAEIIKPVF
LDEKNGKFKIISFYAKKARGL(MSE)SRFIIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR

MGSSHHHHHHSSGLVPRGSHMLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPPQISTLMRISDKLAGIN
AARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFDFAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARG
KDLYQFWGDIITNKLNEALAAQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRFII
ENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR

15% PEG 8000, 0.1 M dibasic sodium phosphate, 0.075 M monobasic sodium phosphate, 0.1 M sodium citrate 4.6, 0.1 M NaCl, 3% w/v benzamidine HCl

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

LILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPPQISTLMRISDKLAGINAARFHDWQPDFTPANARQAIL
AFKGDVYTGLQAETFSEDDFDFAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALAA
QGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRFIIENRLTKPEQLTGFNSEGYFFD
EDSSSNGELVFKRYEQR