K428A mutant nuclease domain of the large terminase subunit gp2 of bacterial virus Sf6 with Manganese and beta-thujaplicinol

Two distinct modes of metal ion binding in the nuclease active site of a viral DNA-packaging terminase: insight into the two-metal-ion catalytic mechanism.

MGSSHHHHHHSSGLVPRGSHMAIIKREWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLM
DINEGADWATSLAIEDGADHYLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAPYQAGAWADEVVQGDNVRT
IGDVFRNKRAQFYYALADRLYLTYRAVVHGEYADPDDMLSFDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTAVEMK
QKLGIPSPNLADALMMCMHCPALVREETEIYVPSSSGW

100mM HEPES, pH7.5, 50mM NaCl, 8% PEG8000

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

( CHAIN A AND ( RESID 213:454 OR RESID 601:728 OR RESID 503:503 OR RESID 501:502 ) )

nuclease domain, metal binding site, METAL BINDING PROTEIN

MAIIKREWLEAATDAHKKLGWKAKGAVVSAHDPSDTGPDAKGYASRHGSVVKRIAEGLLMDINEGADWATSLAIEDGADH
YLWDGDGVGAGLRRQTTEAFSGKKITATMFKGSESPFDEDAPYQAGAWADEVVQGDNVRTIGDVFRNKRAQFYYALADRL
YLTYRAVVHGEYADPDDMLSFDKEAIGEKMLEKLFAELTQIQRKFNNNGKLELMTKVEMKQKLGIPSPNLADALMMCMHC
PALVREETEIYVPSSSGW