Structure of mammalian eIF3 in the context of the 43S preinitiation complex

VAL B 211 HAS WRONG CHIRALITY FOR AN L-AMINO ACID PHE B 213 HAS WRONG CHIRALITY FOR AN L-AMINO ACID GLU B 214 HAS WRONG CHIRALITY FOR AN L-AMINO ACID

SUBMISSION BASED ON EXPERIMENTAL DATA FROM EMDB EMD-3057.

MAMMALIAN 43S PREINITIATION COMPLEX BOUND TO DHX29

VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 100, TEMPERATURE- 120, INSTRUMENT- FEI VITROBOT MARK IV,

EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT B

EUKARYOTIC TRANSLATION INITIATION FACTOR 3 SUBUNIT I

EIF3I, EUKARYOTIC TRANSLATION INITIATION FACTOR 3 39 KDA SUBUNIT, EIF-3 39 KDA SUBUNIT, EIF3 P39, EUKARYOTIC INITIATION FACTOR 3

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MLGAVKMEGHESSDWNSYYADTQEAYSSVPVSNMNSGLGSMNSMNTYMTMNTMTTSGNMTPASFNMSYANPGLGAGLSPG
AVAGMPAGSAGAMNSMTAAGVTAMGTALSPGGMGAMGAQPAASMNGLGPYAAAMNPCMSPMAYAPSNLGRSRAGGGGDAK
TFKRSYPHAKPPYSYISLITMAIQQAPSKMLTLSEIYQWIMDLFPYYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGK
GSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAGAPGAGAGGKKAAAVGAQASQAQLGEVAGPAPDTPAGTESPHS
SASPCQEHKRVALGELKGSAAGAALSPPEPAPSPGQQQQQQPGAEPPPEEEPPRPAGAEDAAAEAAAEAGPEPDVRAEPA
EAQEAASSPAEPPATLPAEQGEAPREQARDEPAPASEAEGSAAEAEPRAVENGDADEPSFSDPEDFVDDVSEEELLGDVL
KDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGY
KLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERA
RWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGH
KKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARV
TLMQLPSRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPN
GSKFAVLHGEAPRISVSFYHVKSNGKIELSKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYM
ASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQ
KDRLSQSKASKELVERRRAMMEDFRKYGKAAQELYVGQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEVIPLGSQ
E

MKAIKLTGHERPLTQVKYNKEGDLLFSCSKDSSASVWYSLNGERLGTLDGHTGTIWSIDVDCFTKYCVTGSADYSIKLWD
VSNGQCVATWKSPVPVKRVEFSPCGNYFLAILDNVMKNPGSINIYEIERDSATHELTKVSEEPIHKIITHEGLDAATVAG
WSTKGKYIIAGHKDGKISKYDVSNNYEYVDSIDLHEKSISDMQFSPDLTYFITSSRDTNSFLVDVSTLQVLKKYETDCPL
NTAVITPLKEFIILGGGQEAKDVTTTSANEGKFEARFYHKIFEEEIGRVQGHFGPLNTVAISPQGTSYASGGEDGFIRLH
HFEKSYFDFKYDVEKAAEAKEHMQEAN

THE SAMPLE OF CHAIN B CONTAINS FULL LENGTH EIF3B.

TRANSLATION, EIF3, EIF3 OCTAMER CORE, MAMMALIAN PREINITIATION 34S COMPLEX, EIF3G/I/B, EIF3D