Crystal structure of Green Fluorescent Protein (GFP); S65T, H148D; circular permutant ( 50-51)

{2-[(1R,2R)-1-amino-2-hydroxypropyl]-4-(4-hydroxybenzylidene)-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic acid

Short Hydrogen Bonds and Proton Delocalization in Green Fluorescent Protein (GFP).

MGHHHHHHSSGGKLPVPWPTLVTTL(CRO)VQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGD
TLVNRIELKGTDFKEDGNILGHKLEYNFNSDNVYITADNQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPD
NHYLSTQTVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGE
GDATIGKLTLKFISTT

MGHHHHHHSSGGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRAVVKFEGDTL
VNRIELKGTDFKEDGNILGHKLEYNFNSDNVYITADNQKNGIKANFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSTQTVLSKDPNEKRDHMVLLEFVTAAGITHGMDELYGGTGGSASQGEELFTGVVPILVELDGDVNGHKFSVRGEGEGD
ATIGKLTLKFISTT

50 mM sodium acetate, 100 mM NaCl, 5% (wt/vol) PEG 3550

National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)

GKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDF
KEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKD
PNEKRDHMVLLEFVTAAGITHGMDELY