Calcium-Dependent Protein Kinase from Eimeria tenella

CDPK is a druggable target in the apicomplexan parasite Eimeria

GPGSPAASKSDKLAATPGMFVQHSTAAFSDRYKGQRVLGKGSFGEVILCKDKVTGQEYAVKVISKRQVKQKTDKELLLKE
VELLKKLDHPNIMKLYEFFEDKGYFYLVTEVYTGGELFDEIISRKRFSEVDAARIIRQVLSGITYMHKNKIVHRDLKPEN
LLLENKRKDANIRIIDFGLSTHFESTKKMKDKIGTAYYIAPEVLHGTYDEKCDVWSTGVILYILLSGCPPFNGANEFDIL
KKVEKGKFTFDLPQWKKVSEPAKDLIRKMLAYVPTMRISARDALEHEWLKTTDAATDSIDVPSLESTILNIRQFQGTQKL
AAAALLYMGSKLTTNEETVELNKIFQRMDKNGDGQLDKQELMEGYVELMKLKGEDVSALDQSAIEFEVEQVLDAVDFDKN
GFIEYSEFVTVAMDRKTLLSRQRLERAFGMFDADGSGKISSSELATIFGVSEVDSETWRRVLAEVDRNNDGEVDFEEFRQ
MLLKLCGDTAA

protein buffer: 25 mM HEPES pH 7.0, 5% glycerol, 500 mM NaCl, 2 mM DTT, 175 uM EtCDPK1; crystallization buffer: 100 mM BIS/TRIS pH 6.5, 23% PEG 3350

National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)

Calcium-Dependent Protein Kinase from Eimeria tenella in complex with ADP

AUTHOR STATED THAT THE UNP REFERENCE Q3HNM4 IS INCORRECT AT RESIDUE 410. THE RESIDUE IDENTITY OF 410 SHOULD BE ASP INSTEAD OF ALA BECAUSE THIS RESIDUE IS IN BINDING TO CALCIUM AND CONSERVED ACROSS THE LARGER FAMILY OF CDPKS.

HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES : WITH TLS ADDED

serine/threonine protein kinase, transferase, calcium-binding, ATP-binding

PAASKSDKLAATPGMFVQHSTAAFSDRYKGQRVLGKGSFGEVILCKDKVTGQEYAVKVISKRQVKQKTDKELLLKEVELL
KKLDHPNIMKLYEFFEDKGYFYLVTEVYTGGELFDEIISRKRFSEVDAARIIRQVLSGITYMHKNKIVHRDLKPENLLLE
NKRKDANIRIIDFGLSTHFESTKKMKDKIGTAYYIAPEVLHGTYDEKCDVWSTGVILYILLSGCPPFNGANEFDILKKVE
KGKFTFDLPQWKKVSEPAKDLIRKMLAYVPTMRISARDALEHEWLKTTDAATDSIDVPSLESTILNIRQFQGTQKLAAAA
LLYMGSKLTTNEETVELNKIFQRMDKNGDGQLDKQELMEGYVELMKLKGEDVSALDQSAIEFEVEQVLDAVAFDKNGFIE
YSEFVTVAMDRKTLLSRQRLERAFGMFDADGSGKISSSELATIFGVSEVDSETWRRVLAEVDRNNDGEVDFEEFRQMLLK
LCGDTAA